Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men

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dc.identifier.uri Schmidt, Simone Stahl, Frank Mutz, Kai-Oliver Scheper, Thomas Hahn, Andreas Schuchardt, Jan Philipp 2016-11-09T10:37:57Z 2016-11-09T10:37:57Z 2012
dc.identifier.citation Schmidt, Simone; Stahl, Frank; Mutz, Kai-Oliver; Scheper, Thomas; Hahn, Andreas et al.: Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men. In: Nutrition and Metabolism 9 (2012), 45. DOI:
dc.description.abstract Background: The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3 PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved in oxidative processes. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR). Results: Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases, in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be observed by qRT-PCR analysis. Conclusions: Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3 PUFAs. Further studies combining genetic and metabolic endpoints are needed to verify the regulative effects of n-3 PUFAs in antioxidative gene expression to better understand their beneficial effects in health and disease prevention. eng
dc.description.sponsorship BMBF
dc.language.iso eng
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartofseries Nutrition and Metabolism 9 (2012)
dc.rights CC BY 2.0
dc.subject Antioxidative defence eng
dc.subject Catalase eng
dc.subject Cytochrome P450 enzyme eng
dc.subject Dyslipidemia eng
dc.subject Glutathione eng
dc.subject Heme oxygenase eng
dc.subject Matrix metalloproteinase eng
dc.subject Omega-3 fatty acids eng
dc.subject Oxylipines eng
dc.subject catalase eng
dc.subject cytochrome P450 eng
dc.subject cytochrome P450 1A2 eng
dc.subject docosahexaenoic acid eng
dc.subject gelatinase A eng
dc.subject glutathione reductase eng
dc.subject glutathione transferase eng
dc.subject heme oxygenase 2 eng
dc.subject icosapentaenoic acid eng
dc.subject immunoglobulin enhancer binding protein eng
dc.subject matrix metalloproteinase eng
dc.subject matrix metalloproteinase 25 eng
dc.subject mitogen activated protein kinase eng
dc.subject omega 3 fatty acid eng
dc.subject stromelysin eng
dc.subject superoxide dismutase eng
dc.subject transcriptome eng
dc.subject unclassified drug eng
dc.subject adult eng
dc.subject antioxidant activity eng
dc.subject article eng
dc.subject clinical article eng
dc.subject controlled study eng
dc.subject down regulation eng
dc.subject drug effect eng
dc.subject dyslipidemia eng
dc.subject erythrocyte membrane eng
dc.subject fatty acid analysis eng
dc.subject gene control eng
dc.subject gene expression eng
dc.subject genetic transcription eng
dc.subject heart protection eng
dc.subject human eng
dc.subject male eng
dc.subject microarray analysis eng
dc.subject nucleotide sequence eng
dc.subject oxidative stress eng
dc.subject randomized controlled trial eng
dc.subject real time polymerase chain reaction eng
dc.subject upregulation eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
dc.type article
dc.type Text
dc.relation.issn 1743-7075
dc.bibliographicCitation.volume 9
dc.bibliographicCitation.firstPage 45
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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