Zusammenfassung: | |
Background: Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are largely unknown. The aim of this study was to investigate the effects of n-3 PUFAs on the expression of lipid metabolism-related genes in normo- and dyslipidemic men to unveil potential genes and pathways affecting lipid metabolism. Methods. Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with six fish oil capsules per day, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. The gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction. Results: Several transcription factors (peroxisome proliferator-activated receptor α (PPARα), retinoid X receptor (RXR) α, RXRγ, hepatic nuclear factor (HNF) 6, and HNF1ß) as well as other genes related to triacylglycerol (TG) synthesis or high-density lipoprotein (HDL-C) and cholesterol metabolism (phospholipids transfer protein, ATP-binding cassette sub-family G member 5, 2-acylglycerol O-acyltransferase (MOGAT) 3, MOGAT2, diacylglycerol O-acyltransferase 1, sterol O-acyltransferase 1, apolipoprotein CII, and low-density lipoprotein receptor) were regulated after n-3 PUFA supplementation, especially in dyslipidemic men. Conclusion: Gene expression analyses revealed several possible molecular pathways by which n-3 PUFAs lower the TG level and increase the HDL-C and low-density lipoprotein level, whereupon the regulation of PPARα appear to play a central role. Trial registration. ClinicalTrials.gov (ID: NCT01089231).
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Lizenzbestimmungen: | CC BY 2.0 Unported - https://creativecommons.org/licenses/by/2.0/ |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2012 |
Schlagwörter (englisch): | Dyslipidemia, HNF, Omega-3 fatty acids, PPARα, RXR, TG lowering, 2 acylglycerol o acyltransferase 2, 2 acylglycerol o acyltransferase 3, ABC transporter G5, acyltransferase, apolipoprotein C2, cholesterol acyltransferase, cholesterol acyltransferase 1, diacylglycerol acyltransferase 1, docosahexaenoic acid, fish oil, hepatic nuclear factor 1beta, hepatic nuclear factor 6, high density lipoprotein cholesterol, icosapentaenoic acid, low density lipoprotein receptor, membrane protein, nuclear factor, omega 3 fatty acid, peroxisome proliferator activated receptor alpha, phospholipid transfer protein, retinoid X receptor alpha, retinoid X receptor gamma, triacylglycerol, unclassified drug, article, blood cell, blood sampling, cholesterol metabolism, clinical article, controlled clinical trial, controlled study, diet supplementation, dyslipidemia, gene expression, genome analysis, human, lipid blood level, lipid metabolism, lipogenesis, male, microarray analysis, nucleotide sequence, protein blood level, real time polymerase chain reaction, Adult, Cholesterol, HDL, Dietary Supplements, Dyslipidemias, Fatty Acids, Omega-3, Fish Oils, Gene Expression, Genome, Human, Humans, Lipid Metabolism, Lipids, Lipoproteins, LDL, Male, Microarray Analysis, Middle Aged, PPAR alpha, Triglycerides |
Fachliche Zuordnung (DDC): | 500 | Naturwissenschaften, 570 | Biowissenschaften, Biologie |
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