Polyethylenimine-mediated gene delivery into human bone marrow mesenchymal stem cells from patients

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dc.identifier.uri http://dx.doi.org/10.15488/641
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/665
dc.contributor.author Wang, Weiwei
dc.contributor.author Li, Wenzhong
dc.contributor.author Ou, Lailiang
dc.contributor.author Flick, Eva
dc.contributor.author Mark, Peter
dc.contributor.author Nesselmann, Catharina
dc.contributor.author Lux, Cornelia A.
dc.contributor.author Gatzen, Hanz-Heinrich
dc.contributor.author Kaminski, Alexander
dc.contributor.author Liebold, Andreas
dc.contributor.author Lützow, Karola
dc.contributor.author Lendlein, Andreas
dc.contributor.author Li, Ren-Ke
dc.contributor.author Steinhoff, Gustav
dc.contributor.author Ma, Nan
dc.date.accessioned 2016-11-03T09:29:55Z
dc.date.available 2016-11-03T09:29:55Z
dc.date.issued 2011
dc.identifier.citation Wang, W.; Li, W.; Ou, L.; Flick, Eva; Mark, P. et al.: Polyethylenimine-mediated gene delivery into human bone marrow mesenchymal stem cells from patients. In: Journal of Cellular and Molecular Medicine 15 (2011), Nr. 9, S. 1989-1998. DOI: http://dx.doi.org/10.1111/j.1582-4934.2010.01130.x
dc.description.abstract Transplantation of mesenchymal stem cells (MSCs) derived from adult bone marrow has been proposed as a potential therapeutic approach for post-infarction left ventricular (LV) dysfunction. However, age-related functional decline of stem cells has restricted their clinical benefits after transplantation into the infarcted myocardium. The limitations imposed on patient cells could be addressed by genetic modification of stem cells. This study was designed to improve our understanding of genetic modification of human bone marrow derived mesenchymal stem cells (hMSCs) by polyethylenimine (PEI, branched with Mw 25 kD), one of non-viral vectors that show promise in stem cell genetic modification, in the context of cardiac regeneration for patients. We optimized the PEI-mediated reporter gene transfection into hMSCs, evaluated whether transfection efficiency is associated with gender or age of the cell donors, analysed the influence of cell cycle on transfection and investigated the transfer of therapeutic vascular endothelial growth factor gene (VEGF). hMSCs were isolated from patients with cardiovascular disease aged from 41 to 85 years. Optimization of gene delivery to hMSCs was carried out based on the particle size of the PEI/DNA complexes, N/P ratio of complexes, DNA dosage and cell viability. The highest efficiency with the cell viability near 60% was achieved at N/P ratio 2 and 6.0 μg DNA/cm 2. The average transfection efficiency for all tested samples, middle-age group (<65 years), old-age group (>65 years), female group and male group was 4.32%, 3.85%, 4.52%, 4.14% and 4.38%, respectively. The transfection efficiency did not show any correlation either with the age or the gender of the donors. Statistically, there were two subpopulations in the donors; and transfection efficiency in each subpopulation was linearly related to the cell percentage in S phase. No significant phenotypic differences were observed between these two subpopulations. Furthermore, PEI-mediated therapeutic gene VEGF transfer could significantly enhance the expression level. eng
dc.description.sponsorship DFG/SFB/Transregio 37
dc.description.sponsorship BMBF/0313191
dc.description.sponsorship German Helmholtz Association
dc.description.sponsorship DFG/0402710
dc.description.sponsorship Ministry of Education/0312138 A
dc.description.sponsorship Ministry of Economy (Mecklenburg-West Pommerania)/V220-630-08-TFMVF/S-035
dc.description.sponsorship Marie Curie International Research Staff Exchange Scheme (IRSES, FP7-PEOPLE-2009-IRSES)
dc.description.sponsorship Reference and Translation Center for Cardiac Stem Cell Therapy (RTC)
dc.language.iso eng
dc.publisher Chichester : Wiley-Blackwell Publishing Ltd.
dc.relation.ispartofseries Journal of Cellular and Molecular Medicine 15 (2011), Nr. 9
dc.rights CC BY 3.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject Cardiovascular disease eng
dc.subject Gene delivery eng
dc.subject Human bone marrow derived mesenchymal stem cells eng
dc.subject Non-viral vector eng
dc.subject Polyethylenimine eng
dc.subject DNA eng
dc.subject green fluorescent protein eng
dc.subject polyethyleneimine eng
dc.subject vasculotropin A eng
dc.subject VEGFA protein, human eng
dc.subject adult eng
dc.subject aged eng
dc.subject article eng
dc.subject bone marrow cell eng
dc.subject cell cycle S phase eng
dc.subject cell death eng
dc.subject cell survival eng
dc.subject cytology eng
dc.subject drug effect eng
dc.subject female eng
dc.subject gene transfer eng
dc.subject genetic transfection eng
dc.subject human eng
dc.subject male eng
dc.subject mesenchymal stem cell eng
dc.subject metabolism eng
dc.subject middle aged eng
dc.subject phenotype eng
dc.subject Adult eng
dc.subject Aged eng
dc.subject Aged, 80 and over eng
dc.subject Bone Marrow Cells eng
dc.subject Cell Death eng
dc.subject Cell Survival eng
dc.subject DNA eng
dc.subject Female eng
dc.subject Gene Transfer Techniques eng
dc.subject Green Fluorescent Proteins eng
dc.subject Humans eng
dc.subject Male eng
dc.subject Mesenchymal Stem Cells eng
dc.subject Middle Aged eng
dc.subject Phenotype eng
dc.subject Polyethyleneimine eng
dc.subject S Phase eng
dc.subject Transfection eng
dc.subject Vascular Endothelial Growth Factor A eng
dc.subject.ddc 600 | Technik ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Polyethylenimine-mediated gene delivery into human bone marrow mesenchymal stem cells from patients
dc.type Article
dc.type Text
dc.relation.issn 1582-1838
dc.relation.doi http://dx.doi.org/10.1111/j.1582-4934.2010.01130.x
dc.bibliographicCitation.issue 9
dc.bibliographicCitation.volume 15
dc.bibliographicCitation.firstPage 1989
dc.bibliographicCitation.lastPage 1998
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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