Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates

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dc.identifier.uri http://dx.doi.org/10.15488/633
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/657
dc.contributor.author Krawinkel, Judith
dc.contributor.author Richter, Undine
dc.contributor.author Torres-Mapa, Maria Leilani
dc.contributor.author Westermann, Martin
dc.contributor.author Gamrad, Lisa
dc.contributor.author Rehbock, Christoph
dc.contributor.author Barcikowski, Stephan
dc.contributor.author Heisterkamp, Alexander
dc.date.accessioned 2016-11-03T09:29:49Z
dc.date.available 2016-11-03T09:29:49Z
dc.date.issued 2016
dc.identifier.citation Krawinkel, J.; Richter, U.; Torres-Mapa, Maria Leilani; Westermann, M.; Gamrad, L. et al.: Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates. In: Journal of Nanobiotechnology 14 (2016), Nr. 1, 2. DOI: http://dx.doi.org/10.1186/s12951-015-0155-8
dc.description.abstract Background: Cell-penetrating peptides (CPPs) can act as carriers for therapeutic molecules such as drugs and genetic constructs for medical applications. The triggered release of the molecule into the cytoplasm can be crucial to its effective delivery. Hence, we implemented and characterized laser interaction with defined gold nanoparticle agglomerates conjugated to CPPs which enables efficient endosomal rupture and intracellular release of molecules transported. Results: Gold nanoparticles generated by pulsed laser ablation in liquid were conjugated with CPPs forming agglomerates and the intracellular release of molecules was triggered via pulsed laser irradiation (λ = 532 nm, τpulse = 1 ns). The CPPs enhance the uptake of the agglomerates along with the cargo which can be co-incubated with the agglomerates. The interaction of incident laser light with gold nanoparticle agglomerates leads to heat deposition and field enhancement in the vicinity of the particles. This highly precise effect deagglomerates the nanoparticles and disrupts the enclosing endosomal membrane. Transmission electron microscopy images confirmed this rupture for radiant exposures of 25 mJ/cm2 and above. Successful intracellular release was shown using the fluorescent dye calcein. For a radiant exposure of 35 mJ/cm2 we found calcein delivery in 81 % of the treated cells while maintaining a high percentage of cell viability. Furthermore, cell proliferation and metabolic activity were not reduced 72 h after the treatment. Conclusion: CPPs trigger the uptake of the gold nanoparticle agglomerates via endocytosis and co-resident molecules in the endosomes are released by applying laser irradiation, preventing their intraendosomal degradation. Due to the highly localized effect, the cell membrane integrity is not affected. Therefore, this technique can be an efficient tool for spatially and temporally confined intracellular release. The utilization of specifically designed photodispersible gold nanoparticle agglomerates (65 nm) can open novel avenues in imaging and molecule delivery. Due to the induced deagglomeration the primary, small particles (~5 nm) are more likely to be removed from the body. eng
dc.description.sponsorship DFG/Ba3580/10
dc.language.iso eng
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartofseries Journal of Nanobiotechnology 14 (2016), Nr. 1
dc.rights CC BY 4.0
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Cell-penetrating peptides eng
dc.subject Endosomes eng
dc.subject Gold nanoparticles eng
dc.subject Intracellular molecule delivery eng
dc.subject Laser-based release eng
dc.subject Particle agglomerates eng
dc.subject Agglomeration eng
dc.subject Cell proliferation eng
dc.subject Cells eng
dc.subject Cytology eng
dc.subject Electron microscopy eng
dc.subject Fiber optic sensors eng
dc.subject Gold eng
dc.subject High resolution transmission electron microscopy eng
dc.subject Irradiation eng
dc.subject Laser ablation eng
dc.subject Medical applications eng
dc.subject Metal nanoparticles eng
dc.subject Molecular biology eng
dc.subject Molecules eng
dc.subject Nanoparticles eng
dc.subject Peptides eng
dc.subject Transmission electron microscopy eng
dc.subject Cell-penetrating peptide eng
dc.subject Endosomes eng
dc.subject Gold Nanoparticles eng
dc.subject Intracellular molecule delivery eng
dc.subject Laser-based eng
dc.subject Particle agglomerates eng
dc.subject Pulsed lasers eng
dc.subject calcein eng
dc.subject cell penetrating peptide eng
dc.subject gold nanoparticle eng
dc.subject animal cell eng
dc.subject Article eng
dc.subject cell proliferation eng
dc.subject cell viability eng
dc.subject cellular distribution eng
dc.subject conjugation eng
dc.subject controlled study eng
dc.subject cytoplasm eng
dc.subject drug cytotoxicity eng
dc.subject electron microscopy eng
dc.subject endocytosis eng
dc.subject endosome eng
dc.subject laser eng
dc.subject membrane damage eng
dc.subject microscopy eng
dc.subject nonhuman eng
dc.subject transmission electron microscopy eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates
dc.type article
dc.type Text
dc.relation.issn 1477-3155
dc.relation.doi http://dx.doi.org/10.1186/s12951-015-0155-8
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 14
dc.bibliographicCitation.firstPage 2
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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