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dc.identifier.uri Bussell, John D. Behrens, Christof Ecke, Wiebke Eubel, Holger 2015-08-24T14:05:33Z 2015-08-24T14:05:33Z 2013-04-24
dc.identifier.citation Bussell, John D.; Behrens, Christof; Ecke, Wiebke; Eubel, Holger: Arabidopsis peroxisome proteomics. In: Frontiers in Plant Science 4 (2013). DOI:
dc.description.abstract The analytical depth of investigation of the peroxisomal proteome of the model plant Arabidopsis thaliana has not yet reached that of other major cellular organelles such as chloroplasts or mitochondria. This is primarily due to the difficulties associated with isolating and obtaining purified samples of peroxisomes from Arabidopsis. So far only a handful of research groups have been successful in obtaining such fractions. To make things worse, enriched peroxisome fractions frequently suffer from significant organellar contamination, lowering confidence in localization assignment of the identified proteins. As with other cellular compartments, identification of peroxisomal proteins forms the basis for investigations of the dynamics of the peroxisomal proteome. It is therefore not surprising that, in terms of functional analyses by proteomic means, peroxisomes are lagging considerably behind chloroplasts or mitochondria. Alternative strategies are needed to overcome the obstacle of hard-to-obtain organellar fractions. This will help to close the knowledge gap between peroxisomes and other organelles and provide a full picture of the physiological pathways shared between organelles. In this review, we briefly summarize the status quo and discuss some of the methodological alternatives to classic organelle proteomic approaches. eng
dc.description.sponsorship Australian ResearchCouncil/CE0561495
dc.description.sponsorship DFG
dc.language.iso eng eng
dc.publisher Lausanne : Frontiers Research Foundation
dc.rights CC BY 3.0 Unported
dc.subject Peroxisome eng
dc.subject Subcellular localization eng
dc.subject Protein:protein interaction eng
dc.subject Free-flow electrophoresis eng
dc.subject Functional proteomics eng
dc.subject Targeted quantitation of proteins eng
dc.subject Arabidopsis thaliana eng
dc.subject Arabidopsis thaliana ger
dc.subject Peroxisomen ger
dc.subject Subzelluläre Lokalisation ger
dc.subject Protein-Protein-Wechselwirkung ger
dc.subject Protein-Protein-Interaktion ger
dc.subject Free-flow-Elektrophorese ger
dc.subject Freifluss-Elektrophorese ger
dc.subject Trägerfreie Elektrophorese ger
dc.subject Funktionelle Proteomik ger
dc.subject Proteinquantifizierung ger
dc.subject Gezielte Proteinquantifizierung ger
dc.subject Proteomanalyse ger
dc.subject.classification Ackerschmalwand ger
dc.subject.classification Peroxisom ger
dc.subject.classification Protein-Protein-Wechselwirkung ger
dc.subject.classification Freie Elektrophorese ger
dc.subject.classification Proteomanalyse ger
dc.subject.classification Quantifizierung ger
dc.subject.classification Protein ger
dc.subject.ddc 580 | Pflanzen (Botanik) ger
dc.title Arabidopsis peroxisome proteomics eng
dc.type Article
dc.type Text
dc.relation.issn 1664-462X
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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