dc.identifier.uri |
http://dx.doi.org/10.15488/601 |
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dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/625 |
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dc.contributor.author |
Chatterjee, Debanjana
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dc.contributor.author |
Marquardt, Nicole
|
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dc.contributor.author |
Tufa, Dejene Milkessa
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dc.contributor.author |
Hatlapatka, Tim
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dc.contributor.author |
Hass, Ralf
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dc.contributor.author |
Kasper, Cornelia
|
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dc.contributor.author |
Kaisenberg, Constantin von
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dc.contributor.author |
Schmidt, Reinhold Ernst
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dc.contributor.author |
Jacobs, Roland
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dc.date.accessioned |
2016-10-31T13:37:21Z |
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dc.date.available |
2016-10-31T13:37:21Z |
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dc.date.issued |
2014 |
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dc.identifier.citation |
Chatterjee, D.; Marquardt, N.; Tufa, D.M.; Hatlapatka, Tim; Hass, R.: Human umbilical cord-derived mesenchymal stem cells utilise activin-A to suppress interferon-gamma production by natural killer cells. In: Frontiers in Immunology 5 (2014), 662. DOI: http://dx.doi.org/10.3389/fimmu.2014.00662 |
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dc.description.abstract |
Following allogeneic hematopoietic stem cell transplantation (HSCT), interferon (IFN)-γ levels in the recipient's body can strongly influence the clinical outcome. Human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are lucrative as biological tolerance inducers in HSCT settings. Hence, we studied the molecular mechanism of how UC-MSCs influence natural killer (NK) cell-mediated IFN-γ production. Allogeneic NK cells were cultured in direct contact with UC-MSCs or cell free supernatants from MSC cultures (MSC conditioned media). We found that soluble factors secreted by UC-MSCs strongly suppressed IL-12/IL-18-induced IFN-γ production by NK cells by reducing phosphorylation of STAT4, NF-κB as well as T-bet activity. UC-MSCs secreted considerable amounts of Activin-A, which could suppress IFN-γ production by NK cells. Neutralisation of Activin-A in MSC conditioned media significantly abrogated their suppressive abilities. Till date, multiple groups have reported that prostaglandin (PG)-E2 produced by MSCs can suppress NK cell functions. Indeed, we found that inhibition of PGE2 production by MSCs could also significantly restore IFN-γ production. However, the effects of Activin-A and PGE2 were not cumulative. To the best of our knowledge, we are first to report the role of Activin-A in MSC mediated suppression of IFN-γ production by NK cells. |
eng |
dc.description.sponsorship |
DFG/SFB738/A5 |
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dc.description.sponsorship |
Hannover Biomedical Research School (HBRS) |
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dc.description.sponsorship |
DFG/REBIRTH |
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dc.description.sponsorship |
Niedersächsische Krebsgesellschaft |
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dc.language.iso |
eng |
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dc.publisher |
Lausanne : Frontiers Research Foundation |
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dc.relation.ispartofseries |
Frontiers in Immunology 5 (2014) |
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dc.rights |
CC BY 4.0 Unported |
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dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
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dc.subject |
Activin-A |
eng |
dc.subject |
IFN-γ production |
eng |
dc.subject |
NK cell |
eng |
dc.subject |
Suppression |
eng |
dc.subject |
T-bet |
eng |
dc.subject |
UC-MSC |
eng |
dc.subject |
5' nucleotidase |
eng |
dc.subject |
activin A |
eng |
dc.subject |
CD14 antigen |
eng |
dc.subject |
CD19 antigen |
eng |
dc.subject |
CD34 antigen |
eng |
dc.subject |
CD45 antigen |
eng |
dc.subject |
endoglin |
eng |
dc.subject |
gamma interferon |
eng |
dc.subject |
HLA DR antigen |
eng |
dc.subject |
immunoglobulin enhancer binding protein |
eng |
dc.subject |
indometacin |
eng |
dc.subject |
interleukin 12 |
eng |
dc.subject |
interleukin 18 |
eng |
dc.subject |
prostaglandin E2 |
eng |
dc.subject |
STAT4 protein |
eng |
dc.subject |
Thy 1 antigen |
eng |
dc.subject |
transcription factor RelA |
eng |
dc.subject |
transcription factor T bet |
eng |
dc.subject |
adipogenesis |
eng |
dc.subject |
Article |
eng |
dc.subject |
bone development |
eng |
dc.subject |
cell differentiation |
eng |
dc.subject |
chondrogenesis |
eng |
dc.subject |
DNA binding |
eng |
dc.subject |
down regulation |
eng |
dc.subject |
enzyme linked immunosorbent assay |
eng |
dc.subject |
flow cytometry |
eng |
dc.subject |
fluorescence activated cell sorting |
eng |
dc.subject |
human |
eng |
dc.subject |
human cell |
eng |
dc.subject |
infant |
eng |
dc.subject |
mesenchymal stem cell |
eng |
dc.subject |
natural killer cell |
eng |
dc.subject |
peripheral blood mononuclear cell |
eng |
dc.subject |
protein expression |
eng |
dc.subject |
protein phosphorylation |
eng |
dc.subject |
umbilical cord derived mesenchymal stem cell |
eng |
dc.subject.ddc |
610 | Medizin, Gesundheit
|
ger |
dc.title |
Human umbilical cord-derived mesenchymal stem cells utilise activin-A to suppress interferon-gamma production by natural killer cells |
|
dc.type |
Article |
|
dc.type |
Text |
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dc.relation.issn |
1664-3224 |
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dc.relation.doi |
http://dx.doi.org/10.3389/fimmu.2014.00662 |
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dc.bibliographicCitation.issue |
DEC |
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dc.bibliographicCitation.volume |
5 |
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dc.bibliographicCitation.firstPage |
662 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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