Evolution of the Rdr1 TNL-cluster in roses and other Rosaceous species

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dc.identifier.uri http://dx.doi.org/10.15488/596
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/620
dc.contributor.author Terefe-Ayana, Diro
dc.contributor.author Kaufmann, Helgard
dc.contributor.author Linde, Marcus
dc.contributor.author Debener, Thomas
dc.date.accessioned 2016-10-31T13:37:19Z
dc.date.available 2016-10-31T13:37:19Z
dc.date.issued 2012
dc.identifier.citation Terefe-Ayana, Diro; Kaufmann, Helgard; Linde, Marcus; Debener, Thomas: Evolution of the Rdr1 TNL-cluster in roses and other Rosaceous species. In: BMC Genomics 13 (2012), Nr. 1, 409. DOI: http://dx.doi.org/10.1186/1471-2164-13-409
dc.description.abstract Background: The resistance of plants to pathogens relies on two lines of defense: a basal defense response and a pathogen-specific system, in which resistance (R) genes induce defense reactions after detection of pathogen-associated molecular patterns (PAMPS). In the specific system, a so-called arms race has developed in which the emergence of new races of a pathogen leads to the diversification of plant resistance genes to counteract the pathogens' effect. The mechanism of resistance gene diversification has been elucidated well for short-lived annual species, but data are mostly lacking for long-lived perennial and clonally propagated plants, such as roses. We analyzed the rose black spot resistance gene, Rdr1, in five members of the Rosaceae: Rosa multiflora, Rosa rugosa, Fragaria vesca (strawberry), Malus x domestica (apple) and Prunus persica (peach), and we present the deduced possible mechanism of R-gene diversification.Results: We sequenced a 340.4-kb region from R. rugosa orthologous to the Rdr1 locus in R. multiflora. Apart from some deletions and rearrangements, the two loci display a high degree of synteny. Additionally, less pronounced synteny is found with an orthologous locus in strawberry but is absent in peach and apple, where genes from the Rdr1 locus are distributed on two different chromosomes. An analysis of 20 TIR-NBS-LRR (TNL) genes obtained from R. rugosa and R. multiflora revealed illegitimate recombination, gene conversion, unequal crossing over, indels, point mutations and transposable elements as mechanisms of diversification.A phylogenetic analysis of 53 complete TNL genes from the five Rosaceae species revealed that with the exception of some genes from apple and peach, most of the genes occur in species-specific clusters, indicating that recent TNL gene diversification began prior to the split of Rosa from Fragaria in the Rosoideae and peach from apple in the Spiraeoideae and continued after the split in individual species. Sequence similarity of up to 99% is obtained between two R. multiflora TNL paralogs, indicating a very recent duplication.Conclusions: The mechanisms by which TNL genes from perennial Rosaceae diversify are mainly similar to those from annual plant species. However, most TNL genes appear to be of recent origin, likely due to recent duplications, supporting the hypothesis that TNL genes in woody perennials are generally younger than those from annuals. This recent origin might facilitate the development of new resistance specificities, compensating for longer generation times in woody perennials. eng
dc.description.sponsorship DFG/DE 511/4-1
dc.description.sponsorship DFG/DE 511/4-2
dc.language.iso eng
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartofseries BMC Genomics 13 (2012), Nr. 1
dc.rights CC BY 2.0
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject apple eng
dc.subject article eng
dc.subject chromosome analysis eng
dc.subject controlled study eng
dc.subject Fragaria eng
dc.subject Fragaria vesca eng
dc.subject gene cluster eng
dc.subject gene conversion eng
dc.subject gene deletion eng
dc.subject gene duplication eng
dc.subject gene locus eng
dc.subject gene rearrangement eng
dc.subject gene sequence eng
dc.subject genetic recombination eng
dc.subject genetic similarity eng
dc.subject genetic variability eng
dc.subject nonhuman eng
dc.subject nucleotide sequence eng
dc.subject peach eng
dc.subject phylogeny eng
dc.subject plant gene eng
dc.subject point mutation eng
dc.subject promoter region eng
dc.subject R gene eng
dc.subject Rdr1 gene eng
dc.subject Rosa multiflora eng
dc.subject Rosa rugosa eng
dc.subject Rosaceae eng
dc.subject rose eng
dc.subject Rosoideae eng
dc.subject sequence alignment eng
dc.subject species difference eng
dc.subject Spiraeoideae eng
dc.subject strawberry eng
dc.subject TNL gene eng
dc.subject transposon eng
dc.subject Chromosomes eng
dc.subject Cluster Analysis eng
dc.subject Contig Mapping eng
dc.subject Evolution, Molecular eng
dc.subject Fragaria eng
dc.subject Genes, Plant eng
dc.subject Genetic Loci eng
dc.subject Malus eng
dc.subject Phylogeny eng
dc.subject Plant Proteins eng
dc.subject Prunus eng
dc.subject Repressor Proteins eng
dc.subject Rosa eng
dc.subject Fragaria eng
dc.subject Fragaria vesca eng
dc.subject Fragaria x ananassa eng
dc.subject Malus x domestica eng
dc.subject Prunus persica eng
dc.subject Rosa eng
dc.subject Rosa multiflora eng
dc.subject Rosa rugosa eng
dc.subject Rosaceae eng
dc.subject Rosoideae eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Evolution of the Rdr1 TNL-cluster in roses and other Rosaceous species
dc.type article
dc.type Text
dc.relation.issn 1471-2164
dc.relation.doi http://dx.doi.org/10.1186/1471-2164-13-409
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 13
dc.bibliographicCitation.firstPage 409
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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