dc.identifier.uri |
http://dx.doi.org/10.15488/579 |
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dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/603 |
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dc.contributor.author |
Khetarpal, Niyati
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dc.contributor.author |
Poddar, Ankur
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dc.contributor.author |
Nemani, Satish K.
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dc.contributor.author |
Dhar, Nisha
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dc.contributor.author |
Patil, Aravind
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dc.contributor.author |
Negi, Priyanka
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dc.contributor.author |
Perween, Ashiya
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dc.contributor.author |
Viswanathan, Ramaswamy
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dc.contributor.author |
Lünsdorf, Heinrich
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dc.contributor.author |
Tyagi, Poornima
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dc.contributor.author |
Raut, Rajendra
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dc.contributor.author |
Arora, Upsana
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dc.contributor.author |
Jain, Swatantra K.
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dc.contributor.author |
Rinas, Ursula
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dc.contributor.author |
Swaminathan, Sathyamangalam
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dc.contributor.author |
Khanna, Navin
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dc.date.accessioned |
2016-10-31T07:59:01Z |
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dc.date.available |
2016-10-31T07:59:01Z |
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dc.date.issued |
2013 |
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dc.identifier.citation |
Khetarpal N.; Poddar, A.; Nemani, Satish K.; Dhar, N.; Patil, A. et al.: Dengue-specific subviral nanoparticles: Design, creation and characterization. In: Journal of Nanobiotechnology 11 (2013), Nr. 1 , 15. DOI: http://dx.doi.org/10.1186/1477-3155-11-15 |
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dc.description.abstract |
Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications. |
eng |
dc.description.sponsorship |
Council of Scientific & Industrial Research, Government of India |
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dc.description.sponsorship |
Department of Biotechnology, Government of India |
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dc.language.iso |
eng |
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dc.publisher |
London : BioMed Central Ltd. |
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dc.relation.ispartofseries |
Journal of Nanobiotechnology 11 (2013), Nr. 1 |
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dc.rights |
CC BY 2.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/2.0/ |
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dc.subject |
Bionanoparticles |
eng |
dc.subject |
Dengue envelope domain III |
eng |
dc.subject |
Hepatitis B surface antigen |
eng |
dc.subject |
Pichia pastoris |
eng |
dc.subject |
Virus-like particle |
eng |
dc.subject |
Bionanoparticles |
eng |
dc.subject |
Envelope domains |
eng |
dc.subject |
Hepatitis B surface antigen |
eng |
dc.subject |
Pichia Pastoris |
eng |
dc.subject |
Virus-like particles |
eng |
dc.subject |
Antigens |
eng |
dc.subject |
Diagnosis |
eng |
dc.subject |
Medical applications |
eng |
dc.subject |
Viruses |
eng |
dc.subject |
antigen |
eng |
dc.subject |
hepatitis B surface antigen |
eng |
dc.subject |
nanoparticle |
eng |
dc.subject |
s antigen |
eng |
dc.subject |
unclassified drug |
eng |
dc.subject |
virus envelope protein |
eng |
dc.subject |
cell extract |
eng |
dc.subject |
hybrid protein |
eng |
dc.subject |
nanoparticle |
eng |
dc.subject |
S envelope protein, hepatitis B virus |
eng |
dc.subject |
virus antigen |
eng |
dc.subject |
virus protein |
eng |
dc.subject |
article |
eng |
dc.subject |
cell lysate |
eng |
dc.subject |
Dengue virus |
eng |
dc.subject |
gene dosage |
eng |
dc.subject |
gene expression |
eng |
dc.subject |
molecular cloning |
eng |
dc.subject |
nonhuman |
eng |
dc.subject |
Pichia pastoris |
eng |
dc.subject |
protein domain |
eng |
dc.subject |
virus like agent |
eng |
dc.subject |
animal |
eng |
dc.subject |
chemistry |
eng |
dc.subject |
Chlorocebus aethiops |
eng |
dc.subject |
dengue |
eng |
dc.subject |
Dengue virus |
eng |
dc.subject |
isolation and purification |
eng |
dc.subject |
metabolism |
eng |
dc.subject |
physiology |
eng |
dc.subject |
Pichia |
eng |
dc.subject |
protein tertiary structure |
eng |
dc.subject |
species difference |
eng |
dc.subject |
ultrastructure |
eng |
dc.subject |
Vero cell line |
eng |
dc.subject |
virion |
eng |
dc.subject |
virology |
eng |
dc.subject |
Dengue virus |
eng |
dc.subject |
Hepatitis B virus |
eng |
dc.subject |
Pichia pastoris |
eng |
dc.subject |
Animals |
eng |
dc.subject |
Antigens, Viral |
eng |
dc.subject |
Cell Extracts |
eng |
dc.subject |
Cercopithecus aethiops |
eng |
dc.subject |
Dengue |
eng |
dc.subject |
Dengue Virus |
eng |
dc.subject |
Nanoparticles |
eng |
dc.subject |
Pichia |
eng |
dc.subject |
Protein Structure, Tertiary |
eng |
dc.subject |
Recombinant Fusion Proteins |
eng |
dc.subject |
Species Specificity |
eng |
dc.subject |
Vero Cells |
eng |
dc.subject |
Viral Envelope Proteins |
eng |
dc.subject |
Viral Proteins |
eng |
dc.subject |
Virion |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.subject.ddc |
610 | Medizin, Gesundheit
|
ger |
dc.title |
Dengue-specific subviral nanoparticles: Design, creation and characterization |
eng |
dc.type |
Article |
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dc.type |
Text |
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dc.relation.issn |
1477-3155 |
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dc.relation.doi |
http://dx.doi.org/10.1186/1477-3155-11-15 |
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dc.bibliographicCitation.issue |
1 |
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dc.bibliographicCitation.volume |
11 |
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dc.bibliographicCitation.firstPage |
15 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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