Browsing Fakultät für Maschinenbau by Author "716528cc-8cb6-4f9e-9877-878c14004473"

Browsing Fakultät für Maschinenbau by Author "716528cc-8cb6-4f9e-9877-878c14004473"

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  • Cooling dynamics of droplets exposed to solid surface freezing and vitrification 
    Liu, Dejia; Oldenhof, Harriëtte; Luo, Xing; Braun, Tobias; Sieme, Harald; Wolkers, Willem F. (Amsterdam [u.a.] : Elsevier, 2024)
    Solid surface freezing or vitrification (SSF/SSV) can be done by depositing droplets of a sample, e.g., cells in a preservation solution, onto a pre-cooled metal surface. It is used to achieve higher cooling rates and ...
  • Freeze-drying of mammalian cells using trehalose: Preservation of DNA integrity 
    Zhang, Miao; Oldenhof, Harriette; Sydykov, Bulat; Bigalk, Judith; Sieme, Harald; Wolkers, Willem F. (London : Nature Publishing Group, 2017)
    The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose ...
  • An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/ macrophages 
    Beneke, Valerie; Küster, Fennja; Neehus, Anna-Lena; Hesse, Christina; Lopez-Rodriguez, Elena; Rafiei Hashtchin, Anna; Schott, Juliane Wilhelmine; Walter, Dorothee; Braun, Armin; Wolkers, Willem F.; Ackermann, Mania; Lachmann, Nico (London : Macmillan Publishers Ltd, 2018)
    Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to ...
  • Loading equine oocytes with cryoprotective agents captured with a finite element method model 
    Içli, Sercan; Soleimani, Meisan; Oldenhof, Harriëtte; Sieme, Harald; Wriggers, Peter; Wolkers, Willem F. (London : Nature Publishing Group, 2021)
    Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect ...
  • Membrane stability during biopreservation of blood cells 
    Stoll, Christoph; Wolkers, Willem F. (Basel : S. Karger AG, 2011)
    Storage methods, which can be taken into consideration for red blood cells and platelets, include liquid storage, cryopreservation and freeze-drying. Red blood cells can be hypothermically stored at refrigerated temperatures, ...
  • Storage stability of liposomes stored at elevated subzero temperatures in DMSO/ sucrose mixtures 
    Sydykov, Bulat; Oldenhof, Harriette; Sieme, Harald; Wolkers, Willem F. (San Francisco, CA : Public Library of Science, 2018)
    Cryopreservation of biological materials is predominantly done using liquid nitrogen, and its application involves high maintenance costs and the need for periodical refilling of liquid nitrogen. Stable storage in mechanical ...
  • Towards reduction or substitution of cytotoxic dmso in biobanking of functional bioengineered megakaryocytes 
    Pogozhykh, Denys; Eicke, Dorothee; Gryshkov, Oleksandr; Wolkers, Willem F.; Schulze, Kai; Guzmán, Carlos A.; Blasczyk, Rainer; Figueiredo, Constança (Basel : Molecular Diversity Preservation International (MDPI), 2020)
    Donor platelet transfusion is currently the only efficient treatment of life-threatening thrombocytopenia, but it is highly challenged by immunological, quality, and contamination issues, as well as short shelf life of the ...
  • Use of sucrose to diminish pore formation in freeze-dried heart valves 
    Vásquez-Rivera, Andrés; Oldenhof, Harriette; Dipresa, Daniele; Goecke, Tobias; Kouvaka, Artemis; Will, Fabian; Haverich, Axel; Korossis, Sotirios; Hilfiker, Andres; Wolkers, Willem F. (London : Nature Publishing Group, 2018)
    Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this ...

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