Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines

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dc.identifier.uri Sambale, Franziska Wagner, S. Stahl, Frank Khaydarov, R.R. Scheper, Thomas Bahnemann, Detlef W. 2016-09-29T11:44:59Z 2016-09-29T11:44:59Z 2015
dc.identifier.citation Sambale, F.; Wagner, S.; Stahl, F.; Khaydarov, R. R.; Scheper, T.; Bahnemann, Detlef W.: Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines. In: Journal of Nanomaterials 2015 (2015), 136765. DOI:
dc.description.abstract Silver nanoparticles are widely used for many applications. In this study silver nanoparticles have been tested for their toxic effect on fibroblasts (NIH-3T3), on a human lung adenocarcinoma epithelial cell line (A-549), on PC-12-cells, a rat adrenal pheochromocytoma cell line, and on HEP-G2-cells, a human hepatocellular carcinoma cell line. The viability of the cells cultivated with different concentrations of silver was determined by the MTT assay, a photometric method to determine cell metabolism. Dose-response curves were extrapolated and IC50, total lethal concentration (TLC), and no observable adverse effect concentration (NOAEC) values were calculated for each cell line. As another approach, ECIS (electric-cell-substrate-impedance-sensing) an automated method to monitor cellular behavior in real-time was applied to observe cells cultivated with silver nanoparticles. To identify the type of cell death the membrane integrity was analyzed by measurements of the lactate dehydrogenase releases and by determination of the caspase 3/7 activity. To ensure that the cytotoxic effect of silver nanoparticles is not traced back to the presence of Ag+ ions in the suspension, an Ag+ salt (AgNO3) has been examined at the same concentration of Ag+ present in the silver nanoparticle suspension that is assuming that the Ag particles are completely available as Ag+ ions. eng
dc.description.sponsorship BMBF/03X0069F
dc.language.iso eng
dc.publisher New York : Hindawi Publishing Corporation
dc.relation.ispartofseries Journal of Nanomaterials 2015 (2015)
dc.rights CC BY 3.0 Unported
dc.subject in-vitro cytotoxicity eng
dc.subject textile fabrics eng
dc.subject antibacterial eng
dc.subject nanosilver eng
dc.subject colloids eng
dc.subject growth eng
dc.subject ions eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 540 | Chemie ger
dc.title Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines
dc.type article
dc.type Text
dc.relation.essn 1687-4129
dc.relation.issn 1687-4110
dc.bibliographicCitation.volume 2015
dc.bibliographicCitation.firstPage 136765
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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