Transcription of TIR1-Controlled Genes Can be Regulated within 10 Min by an Auxin-Induced Process. Can TIR1 be the Receptor?

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dc.identifier.uri Labusch, Corinna Effendi, Yunus Fulda, Martin Scherer, Günther F.E. 2016-09-12T13:13:52Z 2016-09-12T13:13:52Z 2016
dc.identifier.citation Labusch, Corinna; Effendi, Yunus; Fulda, Martin; Scherer, Gunther F. E.: Transcription of TIR1-Controlled Genes Can be Regulated within 10 Min by an Auxin-Induced Process. Can TIR1 be the Receptor? In: Frontiers in Plant Science 7 (2016), 995. DOI:
dc.description.abstract ABP1 and TIR1/AFBs are known as auxin receptors. ABP1 is linked to auxin responses several of which are faster than 10 min. TIR1 regulates auxin-induced transcription of early auxin genes also within minutes. We use transcription of such TIR1-dependent genes as indicator of TIR1 activity to show the rapid regulation of TIR1 by exogenous auxin. To this end, we used quantification of transcription of a set of fifteen early auxin-induced reporter genes at t = 10 and t = 30 min to measure this as a TIR1-dependent auxin response. We conducted this study in 22 mutants of auxin transporters (pin5, abcb1, abcb19, and aux1/lax3), protein kinases and phosphatases (ibr5, npr1, cpk3, CPK3-OX, d6pk1, d6pkl1-1, d6pkl3-2, d6pkl1-1/d6pkl2-2, and d6pkl1-1/d6pkl3-2), of fatty acid metabolism (fad2-1, fad6-1, ssi2, lacs4, lacs9, and lacs4/lacs9) and receptors (tir1, tir1/afb2, and tir1/afb3) and compared them to the wild type. After 10 min auxin application, in 18 out of 22 mutants mis-regulated expression of at least one reporter was found, and in 15 mutants transcription of two-to-three out of five selected auxin reporter genes was mis-regulated. After 30 min of auxin application to mutant plants, mis-regulation of reporter genes ranged from one to 13 out of 15 tested reporter genes. Those genes chosen as mutants were themselves not regulated in their expression by auxin for at least 1 h, excluding an influence of TIR1/AFBs on their transcription. The expression of TIR1/AFB genes was also not modulated by auxin for up to 3 h. Together, this excludes a feedback or feedforward of these mutant genes/proteins on TIR1/AFBs output of transcription in this auxin-induced response. However, an auxin-induced response needed an as yet unknown auxin receptor. We suggest that the auxin receptor necessary for the fast auxin-induced transcription modulation could be, instead, ABP1. The alternative hypothesis would be that auxin-induced expression of a protein, initiated by TIR1/AFBs receptors, could initiate these responses and that this unknown protein regulated TIR1/AFB activities within 10 min. eng
dc.description.sponsorship DLR/50WB133
dc.description.sponsorship DFG/Sche207/24-1
dc.language.iso eng
dc.publisher Lausanne : Frontiers Media Sa
dc.relation.ispartofseries Frontiers in Plant Science 7 (2016)
dc.rights CC BY 4.0 Unported
dc.subject AUXIN-BINDING PROTEIN 1 eng
dc.subject early auxin-induced genes eng
dc.subject auxin transport mutants eng
dc.subject auxin receptor mutants eng
dc.subject fatty acid-metabolism mutants eng
dc.subject protein kinase mutants eng
dc.subject protein phosphatase mutants eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Transcription of TIR1-Controlled Genes Can be Regulated within 10 Min by an Auxin-Induced Process. Can TIR1 be the Receptor? eng
dc.type article
dc.type Text
dc.relation.issn 1664-462X
dc.bibliographicCitation.volume 7
dc.bibliographicCitation.firstPage 995
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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