Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting

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dc.identifier.uri http://dx.doi.org/10.15488/5348
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/5395
dc.contributor.author Buch, Anna
dc.contributor.author Müller, Oliver
dc.contributor.author Ivanova, Lyudmila
dc.contributor.author Döhner, Katinka
dc.contributor.author Bialy, Dagmara
dc.contributor.author Bosse, Jens B.
dc.contributor.author Pohlmann, Anja
dc.contributor.author Binz, Anne
dc.contributor.author Hegemann, Maike
dc.contributor.author Nagel, Claus-Henning
dc.contributor.author Koltzenburg, Martin
dc.contributor.author Viejo-Borbolla, Abel
dc.contributor.author Rosenhahn, Bodo
dc.contributor.author Bauerfeind, Rudolf
dc.contributor.author Sodeik, Beate
dc.date.accessioned 2019-09-03T12:13:07Z
dc.date.available 2019-09-03T12:13:07Z
dc.date.issued 2017
dc.identifier.citation Buch, A.; Müller, O.; Ivanova, L.; Döhner, K.; Bialy, D. et al.: Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting. In: PLoS Pathogens 13 (2017), Nr. 12, e1006813. DOI: https://doi.org/10.1371/journal.ppat.1006813
dc.description.abstract Upon reactivation from latency and during lytic infections in neurons, alphaherpesviruses assemble cytosolic capsids, capsids associated with enveloping membranes, and transport vesicles harboring fully enveloped capsids. It is debated whether capsid envelopment of herpes simplex virus (HSV) is completed in the soma prior to axonal targeting or later, and whether the mechanisms are the same in neurons derived from embryos or from adult hosts. We used HSV mutants impaired in capsid envelopment to test whether the inner tegument proteins pUL36 or pUL37 necessary for microtubule-mediated capsid transport were sufficient for axonal capsid targeting in neurons derived from the dorsal root ganglia of adult mice. Such neurons were infected with HSV1-ΔUL20 whose capsids recruited pUL36 and pUL37, with HSV1-ΔUL37 whose capsids associate only with pUL36, or with HSV1-ΔUL36 that assembles capsids lacking both proteins. While capsids of HSV1-ΔUL20 were actively transported along microtubules in epithelial cells and in the somata of neurons, those of HSV1-ΔUL36 and -ΔUL37 could only diffuse in the cytoplasm. Employing a novel image analysis algorithm to quantify capsid targeting to axons, we show that only a few capsids of HSV1-ΔUL20 entered axons, while vesicles transporting gD utilized axonal transport efficiently and independently of pUL36, pUL37, or pUL20. Our data indicate that capsid motility in the somata of neurons mediated by pUL36 and pUL37 does not suffice for targeting capsids to axons, and suggest that capsid envelopment needs to be completed in the soma prior to targeting of herpes simplex virus to the axons, and to spreading from neurons to neighboring cells. eng
dc.language.iso eng
dc.publisher San Francisco, CA : Public Library of Science (PLoS)
dc.relation.ispartofseries PLoS Pathogens 13 (2017), Nr. 12
dc.rights CC BY 4.0
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject UL20 protein, Herpes simplex virus type 1 eng
dc.subject UL36 protein, Human herpesvirus 1 eng
dc.subject UL37 protein, Human herpesvirus 1 eng
dc.subject viral protein eng
dc.subject animal eng
dc.subject axon eng
dc.subject cell culture eng
dc.subject Chlorocebus aethiops eng
dc.subject genetics eng
dc.subject herpes simplex eng
dc.subject host pathogen interaction eng
dc.subject human eng
dc.subject Human alphaherpesvirus 1 eng
dc.subject mouse eng
dc.subject movement (physiology) eng
dc.subject mutation eng
dc.subject nerve cell eng
dc.subject nerve fiber transport eng
dc.subject pathogenicity eng
dc.subject physiology eng
dc.subject spinal ganglion eng
dc.subject transmission electron microscopy eng
dc.subject ultrastructure eng
dc.subject Vero cell line eng
dc.subject virology eng
dc.subject virus capsid eng
dc.subject Animals eng
dc.subject Axonal Transport eng
dc.subject Axons eng
dc.subject Capsid eng
dc.subject Cells, Cultured eng
dc.subject Cercopithecus aethiops eng
dc.subject Ganglia, Spinal eng
dc.subject Herpes Simplex eng
dc.subject Herpesvirus 1, Human eng
dc.subject Host-Pathogen Interactions eng
dc.subject Humans eng
dc.subject Mice eng
dc.subject Microscopy, Electron, Transmission eng
dc.subject Movement eng
dc.subject Mutation eng
dc.subject Neurons eng
dc.subject Vero Cells eng
dc.subject Viral Proteins eng
dc.subject Viral Structural Proteins eng
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting
dc.type article
dc.type Text
dc.relation.issn 1553-7374
dc.relation.doi https://doi.org/10.1371/journal.ppat.1006813
dc.bibliographicCitation.issue 12
dc.bibliographicCitation.volume 13
dc.bibliographicCitation.firstPage e1006813
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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