Gelatin-methacryloyl (GelMA) hydrogels with defined degree of functionalization as a versatile toolkit for 3D cell culture and extrusion bioprinting

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dc.identifier.uri http://dx.doi.org/10.15488/5346
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/5393
dc.contributor.author Pepelanova, Iliyana
dc.contributor.author Kruppa, Katharina
dc.contributor.author Scheper, Thomas
dc.contributor.author Lavrentieva, Antonina
dc.date.accessioned 2019-09-03T11:52:14Z
dc.date.available 2019-09-03T11:52:14Z
dc.date.issued 2018
dc.identifier.citation Pepelanova, I.; Kruppa, K.; Scheper, T.; Lavrentieva, A.: Gelatin-methacryloyl (GelMA) hydrogels with defined degree of functionalization as a versatile toolkit for 3D cell culture and extrusion bioprinting. In: Bioengineering 5 (2018), Nr. 3, 55. DOI: https://doi.org/10.3390/bioengineering5030055
dc.description.abstract Gelatin-methacryloyl (GelMA) is a semi-synthetic hydrogel which consists of gelatin derivatized with methacrylamide and methacrylate groups. These hydrogels provide cells with an optimal biological environment (e.g., RGD motifs for adhesion) and can be quickly photo-crosslinked, which provides shape fidelity and stability at physiological temperature. In the present work, we demonstrated how GelMA hydrogels can be synthesized with a specific degree of functionalization (DoF) and adjusted to the intended application as a three-dimensional (3D) cell culture platform. The focus of this work lays on producing hydrogel scaffolds which provide a cell promoting microenvironment for human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) and are conductive to their adhesion, spreading, and proliferation. The control of mechanical GelMA properties by variation of concentration, DoF, and ultraviolet (UV) polymerization conditions is described. Moreover, hAD-MSC cell viability and morphology in GelMA of different stiffness was evaluated and compared. Polymerized hydrogels with and without cells could be digested in order to release encapsulated cells without loss of viability. We also demonstrated how hydrogel viscosity can be increased by the use of biocompatible additives, in order to enable the extrusion bioprinting of these materials. Taken together, we demonstrated how GelMA hydrogels can be used as a versatile tool for 3D cell cultivation. eng
dc.language.iso eng
dc.publisher Basel : MDPI AG
dc.relation.ispartofseries Bioengineering 5 (2018), Nr. 3
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject 3D cell culture eng
dc.subject Adipose tissue-derived mesenchymal stem cells (AD-MSCs) eng
dc.subject ASCs eng
dc.subject Bioprinting eng
dc.subject GelMA eng
dc.subject Hydrogels eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Gelatin-methacryloyl (GelMA) hydrogels with defined degree of functionalization as a versatile toolkit for 3D cell culture and extrusion bioprinting
dc.type Article
dc.type Text
dc.relation.issn 2306-5354
dc.relation.doi https://doi.org/10.3390/bioengineering5030055
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 5
dc.bibliographicCitation.firstPage 55
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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