Cryopreservation of cells using defined serum-free cryoprotective agents

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dc.identifier.uri Volbers, Jan-Cedric Lauterböck, Lothar Hofmann, Nicola Glasmacher, Birgit 2019-09-03T11:52:14Z 2019-09-03T11:52:14Z 2016
dc.identifier.citation Volbers, J.-C.; Lauterböck, L.; Hofmann, N.; Glasmacher, B.: Cryopreservation of cells using defined serum-free cryoprotective agents. In: Current Directions in Biomedical Engineering 2 (2016), Nr. 1, S. 315-318. DOI:
dc.description.abstract For regenerative purposes, there is a high demand for viable and active cells. A big issue is to have enough viable cells available at any given time. One solution is cryopreservation. In this context, DMSO is used as cryoprotective agent (CPA) along with fetal bovine serum for nutrient supply and stress shielding effects. To use these cells for human clinical studies, it is important to eliminate the serum to prevent foreign immune reactions and virus transmittance and DMSO for its toxic effect. In this study a serum free cryopreservation solution and protocol has been established. The combination of methylcellulose and poloxamer 188 provide the basis for the new CPA. Other additves are -tocopherol, ectoine, prolin and ascorbic acid. The CPAs were examined with 3T3-cells and multipotent stromal cells from the common marmoset monkey (Callithrix jacchus). The cells were preserved with various CPA concentrations, incubation times and different cooling rates. To enable a higher throughput of encouraging conditions a fluorescence microscopy analysis was used. The use of methylcellulose, poloxamer 188 and -tocopherol enables the reduction of DMSO [up to 2.5% (v/v)] and the elimination of serum without viability losses compared to control. eng
dc.language.iso eng
dc.publisher Berlin : Walter de Gruyter
dc.relation.ispartofseries Current Directions in Biomedical Engineering 2 (2016), Nr. 1
dc.rights CC BY-NC-ND 4.0 Unported
dc.subject Antioxidants eng
dc.subject Cryopreservation eng
dc.subject Fluorescence microscopy eng
dc.subject Methylcellulose eng
dc.subject Multipotent stromal cells eng
dc.subject Poloxamer 188 eng
dc.subject Serum free eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Cryopreservation of cells using defined serum-free cryoprotective agents eng
dc.type Article
dc.type Text
dc.relation.issn 2364-5504
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 2
dc.bibliographicCitation.firstPage 315
dc.bibliographicCitation.lastPage 318
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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