Development of an aptamer-based affinity purification method for vascular endothelial growth factor

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dc.identifier.uri http://dx.doi.org/10.15488/4993
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/5037
dc.contributor.author Lönne, Maren
dc.contributor.author Bolten, Svenja Nicolin
dc.contributor.author Lavrentieva, Antonina
dc.contributor.author Stahl, Frank
dc.contributor.author Scheper, Thomas
dc.contributor.author Walter, Johanna-Gabriela
dc.date.accessioned 2019-06-26T06:32:26Z
dc.date.available 2019-06-26T06:32:26Z
dc.date.issued 2015
dc.identifier.citation Lönne, Maren; Bolten, Svenja Nicolin; Lavrentieva, Antonina; Stahl, Frank; Scheper, Thomas et al.: Development of an aptamer-based affinity purification method for vascular endothelial growth factor. In: Biotechnology Reports 8 (2015), S. 16-23. DOI: https://doi.org/10.1016/j.btre.2015.08.006
dc.description.abstract Since aptamers bind their targets with high affinity and specificity, they are promising alternative ligands in protein affinity purification. As aptamers are chemically synthesized oligonucleotides, they can be easily produced in large quantities regarding GMP conditions allowing their application in protein production for therapeutic purposes. Several advantages of aptamers compared to antibodies are described in general within this paper. Here, an aptamer directed against the human Vascular Endothelial Growth Factor (VEGF) was used as affinity ligand for establishing a purification platform for VEGF in small scale. The aptamer was covalently immobilized on magnetic beads in a controlled orientation resulting in a functional active affinity matrix. Target binding was optimized by introduction of spacer molecules and variation of aptamer density. Further, salt-induced target elution was demonstrated as well as VEGF purification from a complex protein mixture proving the specificity of protein-aptamer binding. eng
dc.language.iso eng
dc.publisher Amsterdam : Elsevier B.V.
dc.relation.ispartofseries Biotechnology Reports 8 (2015)
dc.rights CC BY-NC-ND 4.0
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject Affinity separation eng
dc.subject Aptamer immobilization eng
dc.subject Protein purification eng
dc.subject VEGF eng
dc.subject Aptamer eng
dc.subject Oligonucleotide eng
dc.subject Affinity chromatography eng
dc.subject Vascular endothelial growth factor eng
dc.subject Protein biosynthesis eng
dc.subject Biochemistry eng
dc.subject Tandem affinity purification eng
dc.subject Ligand eng
dc.subject Biology eng
dc.subject Protein purification eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Development of an aptamer-based affinity purification method for vascular endothelial growth factor
dc.type article
dc.type Text
dc.relation.issn 2215-017X
dc.relation.doi https://doi.org/10.1016/j.btre.2015.08.006
dc.bibliographicCitation.volume 8
dc.bibliographicCitation.firstPage 16
dc.bibliographicCitation.lastPage 23
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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