Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers

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dc.identifier.uri http://dx.doi.org/10.15488/476
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/500
dc.contributor.author Walter, Johanna-Gabriela
dc.contributor.author Petersen, Svea
dc.contributor.author Stahl, Frank
dc.contributor.author Scheper, Thomas
dc.contributor.author Barcikowski, Stephan
dc.date.accessioned 2016-08-31T09:19:34Z
dc.date.available 2016-08-31T09:19:34Z
dc.date.issued 2010
dc.identifier.citation Walter, Johanna G.; Petersen, Svea; Stahl, Frank; Scheper, Thomas; Barcikowski, Stephan: Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers. In: Journal of Nanobiotechnology 8 (2010), 21. DOI: http://dx.doi.org/10.1186/1477-3155-8-21
dc.description.abstract Background: Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. In this context, in situ conjugation of nanoparticles with biomolecules via laser ablation in an aqueous media is a highly promising one-step method for the production of functional nanoparticles resulting in highly efficient conjugation. Increased yields are required, particularly considering the conjugation of cost-intensive biomolecules like RNA aptamers. Results: Using a DNA aptamer directed against streptavidin, in situ conjugation results in nanoparticles with diameters of approximately 9 nm exhibiting a high aptamer surface density (98 aptamers per nanoparticle) and a maximal conjugation efficiency of 40.3%. We have demonstrated the functionality of the aptamer-conjugated nanoparticles using three independent analytical methods, including an agglomeration-based colorimetric assay, and solid-phase assays proving high aptamer activity. To demonstrate the general applicability of the in situ conjugation of gold nanoparticles with aptamers, we have transferred the method to an RNA aptamer directed against prostate-specific membrane antigen (PSMA). Successful detection of PSMA in human prostate cancer tissue was achieved utilizing tissue microarrays. Conclusions: In comparison to the conventional generation of bio-conjugated gold nanoparticles using chemical synthesis and subsequent bio-functionalization, the laser-ablation-based in situ conjugation is a rapid, one-step production method. Due to high conjugation efficiency and productivity, in situ conjugation can be easily used for high throughput generation of gold nanoparticles conjugated with valuable biomolecules like aptamers. eng
dc.description.sponsorship DFG/REBIRTH
dc.language.iso eng
dc.publisher London : Biomed Central Ltd.
dc.relation.ispartofseries Journal of Nanobiotechnology 8 (2010)
dc.rights CC BY 2.0
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject PSMA eng
dc.subject AuNP eng
dc.subject DNA eng
dc.subject RNA eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers
dc.type article
dc.type Text
dc.relation.issn 1477-3155
dc.relation.doi http://dx.doi.org/10.1186/1477-3155-8-21
dc.bibliographicCitation.volume 8
dc.bibliographicCitation.firstPage 21
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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