MediPlEx - a tool to combine in silico & experimental gene expression profiles of the model legume Medicago truncatula

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dc.identifier.uri http://dx.doi.org/10.15488/471
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/495
dc.contributor.author Henckel, Kolja
dc.contributor.author Küster, Helge
dc.contributor.author Stutz, Leonhard J.
dc.contributor.author Goesmann, Alexander
dc.date.accessioned 2016-08-31T09:19:32Z
dc.date.available 2016-08-31T09:19:32Z
dc.date.issued 2010
dc.identifier.citation Henckel, Kolja; Küster, Helge; Stutz, Leonhard J.; Goesmann, Alexander: MediPlEx - a tool to combine in silico & experimental gene expression profiles of the model legume Medicago truncatula. In: BMC Research Notes 3 (2010), 262. DOI: http://dx.doi.org/10.1186/1756-0500-3-262
dc.description.abstract Background: Expressed Sequence Tags (ESTs) are in general used to gain a first insight into gene activities from a species of interest. Subsequently, and typically based on a combination of EST and genome sequences, microarray-based expression analyses are performed for a variety of conditions. In some cases, a multitude of EST and microarray experiments are conducted for one species, covering different tissues, cell states, and cell types. Under these circumstances, the challenge arises to combine results derived from the different expression profiling strategies, with the goal to uncover novel information on the basis of the integrated datasets. Findings: Using our new analysis tool, MediPlEx (MEDIcago truncatula multiPLe EXpression analysis), expression data from EST experiments, oligonucleotide microarrays and Affymetrix GeneChips® can be combined and analyzed, leading to a novel approach to integrated transcriptome analysis. We have validated our tool via the identification of a set of well-characterized AM-specific and AM-induced marker genes, identified by MediPlEx on the basis of in silico and experimental gene expression profiles from roots colonized with AM fungi. Conclusions: MediPlEx offers an integrated analysis pipeline for different sets of expression data generated for the model legume Medicago truncatula. As expected, in silico and experimental gene expression data that cover the same biological condition correlate well. The collection of differentially expressed genes identified via MediPlEx provides a starting point for functional studies in plant mutants. eng
dc.language.iso eng
dc.publisher London : Biomed Central Ltd.
dc.relation.ispartofseries BMC Research Notes 3 (2010)
dc.rights CC BY 2.0
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject Medicago truncatula eng
dc.subject legume biology eng
dc.subject Expressed Sequence Tags eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title MediPlEx - a tool to combine in silico & experimental gene expression profiles of the model legume Medicago truncatula
dc.type article
dc.type Text
dc.relation.essn 1756-0500
dc.relation.doi http://dx.doi.org/10.1186/1756-0500-3-262
dc.bibliographicCitation.volume 3
dc.bibliographicCitation.firstPage 262
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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