dc.identifier.uri |
http://dx.doi.org/10.15488/4843 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/4886 |
|
dc.contributor.author |
Hartmann, Rico M.
|
|
dc.contributor.author |
Schaepe, Sieke
|
|
dc.contributor.author |
Nübel, Daniel
|
|
dc.contributor.author |
Petersen, Arne C.
|
|
dc.contributor.author |
Bertolini, Martina
|
|
dc.contributor.author |
Vasilev, Jana
|
|
dc.contributor.author |
Küster, Helge
|
|
dc.contributor.author |
Hohnjec, Natalija
|
|
dc.date.accessioned |
2019-05-21T11:52:22Z |
|
dc.date.available |
2019-05-21T11:52:22Z |
|
dc.date.issued |
2019 |
|
dc.identifier.citation |
Hartmann, R.M. et al.: Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis. In: Scientific Reports 9 (2019), 3360. DOI: https://doi.org/10.1038/s41598-019-40214-4 |
|
dc.description.abstract |
To improve access to limiting nutrients, the vast majority of land plants forms arbuscular mycorrhizal (AM) symbioses with Glomeromycota fungi. We show here that AM-related GRAS transcription factors from different subgroups are upregulated during a time course of mycorrhization. Based on expression studies in mutants defective in arbuscule branching (ram1-1, with a deleted MtRam1 GRAS transcription factor gene) or in the formation of functional arbuscules (pt4-2, mutated in the phosphate transporter gene MtPt4), we demonstrate that the five AM-related GRAS transcription factor genes MtGras1, MtGras4, MtGras6, MtGras7, and MtRad1 can be differentiated by their dependency on MtRAM1 and MtPT4, indicating that the network of AM-related GRAS transcription factors consists of at least two regulatory modules. One module involves the MtRAM1- and MtPT4-independent transcription factor MtGRAS4 that activates MtGras7. Another module is controlled by the MtRAM1- and MtPT4-dependent transcription factor MtGRAS1. Genome-wide expression profiles of mycorrhized MtGras1 knockdown and ram1-1 roots differ substantially, indicating different targets. Although an MtGras1 knockdown reduces transcription of AM-related GRAS transcription factor genes including MtRam1 and MtGras7, MtGras1 overexpression alone is not sufficient to activate MtGras genes. MtGras1 knockdown roots display normal fungal colonization, with a trend towards the formation of smaller arbuscules. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Berlin : Springer Nature |
|
dc.relation.ispartofseries |
Scientific Reports 9 (2019) |
|
dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Arbuscular mycorrhiza |
eng |
dc.subject |
Fluorescence imaging |
eng |
dc.subject |
Gene expression profiling |
eng |
dc.subject |
Plant molecular biology |
eng |
dc.subject |
Plant signalling |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.title |
Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis |
|
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
2045-2322 |
|
dc.relation.doi |
https://doi.org/10.1038/s41598-019-40214-4 |
|
dc.bibliographicCitation.volume |
9 |
|
dc.bibliographicCitation.firstPage |
3360 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|