Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis

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dc.identifier.uri Hartmann, Rico M. Schaepe, Sieke Nübel, Daniel Petersen, Arne C. Bertolini, Martina Vasilev, Jana Küster, Helge Hohnjec, Natalija 2019-05-21T11:52:22Z 2019-05-21T11:52:22Z 2019
dc.identifier.citation Hartmann, R.M. et al.: Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis. In: Scientific Reports 9 (2019), 3360. DOI:
dc.description.abstract To improve access to limiting nutrients, the vast majority of land plants forms arbuscular mycorrhizal (AM) symbioses with Glomeromycota fungi. We show here that AM-related GRAS transcription factors from different subgroups are upregulated during a time course of mycorrhization. Based on expression studies in mutants defective in arbuscule branching (ram1-1, with a deleted MtRam1 GRAS transcription factor gene) or in the formation of functional arbuscules (pt4-2, mutated in the phosphate transporter gene MtPt4), we demonstrate that the five AM-related GRAS transcription factor genes MtGras1, MtGras4, MtGras6, MtGras7, and MtRad1 can be differentiated by their dependency on MtRAM1 and MtPT4, indicating that the network of AM-related GRAS transcription factors consists of at least two regulatory modules. One module involves the MtRAM1- and MtPT4-independent transcription factor MtGRAS4 that activates MtGras7. Another module is controlled by the MtRAM1- and MtPT4-dependent transcription factor MtGRAS1. Genome-wide expression profiles of mycorrhized MtGras1 knockdown and ram1-1 roots differ substantially, indicating different targets. Although an MtGras1 knockdown reduces transcription of AM-related GRAS transcription factor genes including MtRam1 and MtGras7, MtGras1 overexpression alone is not sufficient to activate MtGras genes. MtGras1 knockdown roots display normal fungal colonization, with a trend towards the formation of smaller arbuscules. eng
dc.language.iso eng
dc.publisher Berlin : Springer Nature
dc.relation.ispartofseries Scientific Reports 9 (2019)
dc.rights CC BY 4.0 Unported
dc.subject Arbuscular mycorrhiza eng
dc.subject Fluorescence imaging eng
dc.subject Gene expression profiling eng
dc.subject Plant molecular biology eng
dc.subject Plant signalling eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis
dc.type article
dc.type Text
dc.relation.essn 2045-2322
dc.bibliographicCitation.volume 9
dc.bibliographicCitation.firstPage 3360
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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