Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs

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dc.identifier.uri http://dx.doi.org/10.15488/4842
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4885
dc.contributor.author Mücke, Stefanie
dc.contributor.author Reschke, Maik
dc.contributor.author Erkes, Annett
dc.contributor.author Schwietzer, Claudia-Alice
dc.contributor.author Becker, Sebastian
dc.contributor.author Streubel, Jana
dc.contributor.author Morgan, Richard D.
dc.contributor.author Wilson, Geoffrey G.
dc.contributor.author Grau, Jan
dc.contributor.author Boch, Jens
dc.date.accessioned 2019-05-21T10:57:56Z
dc.date.available 2019-05-21T10:57:56Z
dc.date.issued 2019
dc.identifier.citation Mücke, S. et al.: Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs. In: Frontiers in Plant Science 10 (2019), 162. DOI: https://doi.org/10.3389/fpls.2019.00162
dc.description.abstract Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the plant targets of TALEs, we determined the genome sequences of the Indian X. oryzae pv. oryzae (Xoo) type strain ICMP 3125T and the strain PXO142 from the Philippines. Their complete TALE repertoire was analyzed and genome-wide TALE targets in rice were characterized. Integrating computational target predictions and rice transcriptomics data, we were able to verify 12 specifically induced target rice genes. The TALEs of the Xoo strains were reconstructed and expressed in a TALE-free Xoo strain to attribute specific induced genes to individual TALEs. Using reporter assays, we could show that individual TALEs act directly on their target promoters. In particular, we show that TALE classes assigned by AnnoTALE reflect common target genes, and that TALE classes of Xoo and the related pathogen X. oryzae pv. oryzicola share more common target genes than previously believed. Taken together, we establish a detailed picture of TALE-induced plant processes that significantly expands our understanding of X. oryzae virulence strategies and will facilitate the development of novel resistances to overcome this important rice disease. eng
dc.language.iso eng
dc.publisher Lausanne : Frontiers Media
dc.relation.ispartofseries Frontiers in Plant Science 10 (2019)
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject TALE eng
dc.subject Xanthomonas oryzae eng
dc.subject genome eng
dc.subject plant pathogen eng
dc.subject rice eng
dc.subject salicylic acid eng
dc.subject type III effector eng
dc.subject virulence eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs eng
dc.type article
dc.type Text
dc.relation.essn 1664-462X
dc.relation.doi https://doi.org/10.3389/fpls.2019.00162
dc.bibliographicCitation.volume 10
dc.bibliographicCitation.firstPage 162
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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