Real-time PCR quantification and diversity analysis of the functional genes aprA and dsrA of sulfate-reducing prokaryotes in marine sediments of the Peru continental margin and the Black Sea

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dc.identifier.uri http://dx.doi.org/10.15488/464
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/487
dc.contributor.author Blazejak, Anna
dc.contributor.author Schippers, Axel
dc.date.accessioned 2016-08-30T10:20:24Z
dc.date.available 2016-08-30T10:20:24Z
dc.date.issued 2011
dc.identifier.citation Blazejak, Anna; Schippers, Axel: Real-time PCR quantification and diversity analysis of the functional genes aprA and dsrA of sulfate-reducing prokaryotes in marine sediments of the Peru continental margin and the Black Sea. In: Frontiers in Microbiology 2 (2011), 253. DOI: http://dx.doi.org/10.3389/fmicb.2011.00253
dc.description.abstract Sulfate-reducing prokaryotes (SRP) are ubiquitous and quantitatively important members in many ecosystems, especially in marine sediments. However their abundance and diversity in subsurface marine sediments is poorly understood. In this study, the abundance and diversity of the functional genes for the enzymes adenosine 5'-phosphosulfate reductase (aprA) and dissimilatory sulfite reductase (dsrA) of SRP in marine sediments of the Peru continental margin and the Black Sea were analyzed, including samples from the deep biosphere (ODP site 1227). For aprA quantification a Q-PCR assay was designed and evaluated. Depth profiles of the aprA and dsrA copy numbers were almost equal for all sites. Gene copy numbers decreased concomitantly with depth from around 10(8)/g sediment close to the sediment surface to less than 10(5)/g sediment at 5 mbsf. The 16S rRNA gene copy numbers of total bacteria were much higher than those of the functional genes at all sediment depths and used to calculate the proportion of SRP to the total Bacteria. The aprA and dsrA copy numbers comprised in average 0.5-1% of the 16S rRNA gene copy numbers of total bacteria in the sediments up to a depth of ca. 40 mbsf. In the zone without detectable sulfate in the pore water from about 40-121 mbsf (Peru margin ODP site 1227), only dsrA (but not aprA) was detected with copy numbers of less than 10(4)/g sediment, comprising ca. 14% of the 16S rRNA gene copy numbers of total bacteria. In this zone, sulfate might be provided for SRP by anaerobic sulfide oxidation. Clone libraries of aprA showed that all isolated sequences originate from SRP showing a close relationship to aprA of characterized species or form a new cluster with only distant relation to aprA of isolated SRP. For dsrA a high diversity was detected, even up to 121 m sediment depth in the deep biosphere. eng
dc.description.sponsorship DFG/IODP/ODP/SCHI 535/5
dc.language.iso eng
dc.publisher Lausanne : Frontiers Research Foundation
dc.relation.ispartofseries Frontiers in Microbiology 2 (2011)
dc.rights CC BY-NC 3.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc/3.0/
dc.subject deep biosphere eng
dc.subject real-time pcr eng
dc.subject subsurface eng
dc.subject odp eng
dc.subject sulfate-reducing prokaryotes eng
dc.subject apra eng
dc.subject dsra eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Real-time PCR quantification and diversity analysis of the functional genes aprA and dsrA of sulfate-reducing prokaryotes in marine sediments of the Peru continental margin and the Black Sea
dc.type article
dc.type Text
dc.relation.issn 1664-302X
dc.relation.doi http://dx.doi.org/10.3389/fmicb.2011.00253
dc.bibliographicCitation.volume 2
dc.bibliographicCitation.firstPage 253
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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