dc.identifier.uri |
http://dx.doi.org/10.15488/462 |
|
dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/485 |
|
dc.contributor.author |
Lavrentieva, Antonina
|
|
dc.contributor.author |
Majore, Ingrida
|
|
dc.contributor.author |
Kasper, Cornelia
|
|
dc.contributor.author |
Hass, Ralf
|
|
dc.date.accessioned |
2016-08-30T10:20:24Z |
|
dc.date.available |
2016-08-30T10:20:24Z |
|
dc.date.issued |
2010-06-16 |
|
dc.identifier.citation |
Lavrentieva, Antonina; Majore, Ingrida; Kasper, Cornelia; Hass, Ralf: Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells. In: Cell Communication and Signaling 8 (2010) , 18. DOI: http://dx.doi.org/10.1186/1478-811X-8-18 |
|
dc.description.abstract |
Following cultivation of distinct mesenchymal stem cell (MSC) populations derived from human umbilical cord under hypoxic conditions (between 1.5% to 5% oxygen (O-2)) revealed a 2- to 3-fold reduced oxygen consumption rate as compared to the same cultures at normoxic oxygen levels (21% O-2). A simultaneous measurement of dissolved oxygen within the culture media from 4 different MSC donors ranged from 15 mu mol/L at 1.5% O-2 to 196 mu mol/L at normoxic 21% O-2. The proliferative capacity of the different hypoxic MSC populations was elevated as compared to the normoxic culture. This effect was paralleled by a significantly reduced cell damage or cell death under hypoxic conditions as evaluated by the cellular release of LDH whereby the measurement of caspase 3/7 activity revealed little if any differences in apoptotic cell death between the various cultures. The MSC culture under hypoxic conditions was associated with the induction of hypoxia-inducing factor-alpha (HIF-1 alpha) and an elevated expression of energy metabolism-associated genes including GLUT-1, LDH and PDK1. Concomitantly, a significantly enhanced glucose consumption and a corresponding lactate production could be observed in the hypoxic MSC cultures suggesting an altered metabolism of these human stem cells within the hypoxic environment. |
eng |
dc.description.sponsorship |
DFG/EXC/REBIRTH |
|
dc.language.iso |
eng |
|
dc.publisher |
London : Biomed Central Ltd |
|
dc.relation.ispartofseries |
Cell Communication and Signaling 8 (2010) |
|
dc.rights |
CC BY 2.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/2.0/ |
|
dc.subject |
adipose-tissue |
eng |
dc.subject |
bone-marrow |
eng |
dc.subject |
oxygen concentration |
eng |
dc.subject |
reactive oxygen |
eng |
dc.subject |
metabolism |
eng |
dc.subject |
growth |
eng |
dc.subject |
consumption |
eng |
dc.subject |
responses |
eng |
dc.subject |
damage |
eng |
dc.subject |
dna |
eng |
dc.subject.ddc |
500 | Naturwissenschaften
|
ger |
dc.title |
Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells |
|
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.issn |
1478-811X |
|
dc.relation.doi |
http://dx.doi.org/10.1186/1478-811X-8-18 |
|
dc.bibliographicCitation.volume |
8 |
|
dc.bibliographicCitation.firstPage |
18 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|