Anaerobic oxidation of methane at a marine methane seep in a forearc sediment basin off Sumatra, Indian Ocean

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dc.identifier.uri Siegert, Michael Krueger, Martin Teichert, Barbara Wiedicke, Michael Schippers, Axel 2016-08-30T10:20:22Z 2016-08-30T10:20:22Z 2011
dc.identifier.citation Siegert, Michael; Krueger, Martin; Teichert, Barbara; Wiedicke, Michael; Schippers, Axel: Anaerobic oxidation of methane at a marine methane seep in a forearc sediment basin off Sumatra, Indian Ocean. In: Frontiers in Microbiology 2 (2011), 249. DOI:
dc.description.abstract A cold methane seep was discovered in a forearc sediment basin off the island Sumatra, exhibiting a methane-seep adapted microbial community. A defined seep center of activity, like in mud volcanoes, was not discovered. The seep area was rather characterized by a patchy distribution of active spots. The relevance of anaerobic oxidation of methane (AOM) was reflected by C-13-depleted isotopic signatures of dissolved inorganic carbon. The anaerobic conversion of methane to CO2 was confirmed in a C-13-labeling experiment. Methane fueled a vital microbial community with cell numbers of up to 4 x 10(9) cells cm(-3) sediment. The microbial community was analyzed by total cell counting, catalyzed reporter deposition fluorescence in situ hybridization (CARD FISH), quantitative real-time PCR (qPCR), and denaturing gradient gel electrophoresis (DGGE). CARD FISH cell counts and qPCR measurements showed the presence of Bacteria and Archaea, but only small numbers of Eukarya. The archaeal community comprised largely members of ANME-1 and ANME-2. Furthermore, members of the Crenarchaeota were frequently detected in the DGGE analysis. Three major bacterial phylogenetic groups (delta-Proteobacteria, candidate division OP9, and Anaerolineaceae) were abundant across the study area. Several of these sequences were closely related to the genus Desulfococcus of the family Desulfobacteraceae, which is in good agreement with previously described AOM sites. In conclusion, the majority of the microbial community at the seep consisted of AOM-related microorganisms, while the relevance of higher hydrocarbons as microbial substrates was negligible. eng
dc.description.sponsorship BMBF/03G0189A
dc.description.sponsorship DFG/KR 3311/5-1
dc.description.sponsorship DFG/KR 3311/5-2
dc.language.iso eng
dc.publisher Lausanne : Frontiers Research Foundation
dc.relation.ispartofseries Frontiers in Microbiology 2 (2011)
dc.rights CC BY-NC 3.0 Unported
dc.subject dgge eng
dc.subject quantitative pcr eng
dc.subject card-fish eng
dc.subject methane seep eng
dc.subject stable isotopes eng
dc.subject aom eng
dc.subject hydrocarbon-dependent methanogenesis eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Anaerobic oxidation of methane at a marine methane seep in a forearc sediment basin off Sumatra, Indian Ocean eng
dc.type article
dc.type Text
dc.relation.issn 1664-302X
dc.bibliographicCitation.volume 2
dc.bibliographicCitation.firstPage 249
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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