Evolution of Transcription Activator-Like Effectors in Xanthomonas oryzae

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dc.identifier.uri http://dx.doi.org/10.15488/4791
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4834
dc.contributor.author Erkes, Annett
dc.contributor.author Reschke, Maik
dc.contributor.author Boch, Jens
dc.contributor.author Grau, Jan
dc.date.accessioned 2019-05-16T09:34:10Z
dc.date.available 2019-05-16T09:34:10Z
dc.date.issued 2017
dc.identifier.citation Erkes, A.; Reschke, M.; Boch, J.; Grau, J.: Evolution of Transcription Activator-Like Effectors in Xanthomonas oryzae. In: Genome Biology and Evolution 9 (2017), Nr. 6, S. 1599-1615. DOI: https://doi.org/10.1093/gbe/evx108
dc.description.abstract Transcription activator-like effectors (TALEs) are secreted by plant-pathogenic Xanthomonas bacteria into plant cells where they act as transcriptional activators and, hence, are major drivers in reprogramming the plant for the benefit of the pathogen. TALEs possess a highly repetitive DNA-binding domain of typically 34 amino acid(AA) tandem repeats, where AA 12 and 13, termed repeat variable di-residue (RVD), determine target specificity. Different Xanthomonas strains possess different repertoires of TALEs. Here, we study the evolution of TALEs from the level of RVDs determining target specificity down to the level of DNA sequence with focus on rice-pathogenic Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) strains. We observe that codon pairs coding for individual RVDs are conserved to a similar degree as the flanking repeat sequence. We find strong indications that TALEs may evolve 1) by base substitutions in codon pairs coding for RVDs, 2) by recombination of N-terminal or C-terminal regions of existing TALEs, or 3) by deletion of individual TALE repeats, and we propose possible mechanisms. We find indications that the reassortment of TALE genes in clusters is mediated by an integron-like mechanism in Xoc. We finally study the effect of the presence/absence and evolutionary modifications of TALEs on transcriptional activation of putative target genes in rice, and find that even single RVD swaps may lead to considerable differences in activation. This correlation allowed a refined prediction of TALE targets, which is the crucial step to decipher their virulence activity. eng
dc.language.iso eng
dc.publisher Oxford : Oxford University Press
dc.relation.ispartofseries Genome Biology and Evolution 9 (2017), Nr. 6
dc.rights CC BY-NC 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc/4.0/
dc.subject Xanthomonas eng
dc.subject transcription activator-like effectors (TALEs) eng
dc.subject evolution of TALEs eng
dc.subject evolutionary mechanisms eng
dc.subject plant-pathogen interaction eng
dc.subject transcriptional response eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Evolution of Transcription Activator-Like Effectors in Xanthomonas oryzae
dc.type Article
dc.type Text
dc.relation.issn 1759-6653
dc.relation.doi https://doi.org/10.1093/gbe/evx108
dc.bibliographicCitation.issue 6
dc.bibliographicCitation.volume 9
dc.bibliographicCitation.firstPage 1599
dc.bibliographicCitation.lastPage 1615
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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