Quantification of microbial communities in subsurface marine sediments of the Black Sea and off Namibia

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dc.identifier.uri http://dx.doi.org/10.15488/459
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/482
dc.contributor.author Schippers, Axel
dc.contributor.author Kock, Dagmar
dc.contributor.author Hoeft, Carmen
dc.contributor.author Koeweker, Gerrit
dc.contributor.author Siegert, Michael
dc.date.accessioned 2016-08-29T13:44:52Z
dc.date.available 2016-08-29T13:44:52Z
dc.date.issued 2012
dc.identifier.citation Schippers, Axel; Kock, Dagmar; Hoeft, Carmen; Koeweker, Gerrit; Siegert, Michael: Quantification of microbial communities in subsurface marine sediments of the Black Sea and off Namibia. In: Frontiers in Microbiology 3 (2012), 16. DOI: http://dx.doi.org/10.3389/fmicb.2012.00016
dc.description.abstract Organic-rich subsurface marine sediments were taken by gravity coring up to a depth of 10 m below seafloor at six stations from the anoxic Black Sea and the Benguela upwelling system off Namibia during the research cruises Meteor 72-5 and 76-1, respectively. The quantitative microbial community composition at various sediment depths was analyzed using total cell counting, catalyzed reporter deposition fluorescence in situ hybridization (CARD FISH) and quantitative real-time PCR (Q-PCR). Total cell counts decreased with depths from 10(9) to 10(10) cells/mL at the sediment surface to 10(7)-10(9) cells/mL below one meter depth. Based on CARD FISH and Q-PCR analyses overall similar proportions of Bacteria and Archaea were found. The down-core distribution of prokaryotic and eukaryotic small subunit ribosomal RNA genes (16S and 18S rRNA) as well as functional genes involved in different biogeochemical processes was quantified using Q-PCR. Crenarchaeota and the bacterial candidate division JS-1 as well as the classes Anaerolineae and Caldilineae of the phylum Chloroflexi were highly abundant. Less abundant but detectable in most of the samples were Eukarya as well as the metal and sulfate-reducing Geobacteraceae (only in the Benguela upwelling influenced sediments). The functional genes cbbL, encoding for the large subunit of RuBisCO, the genes dsrA and aprA, indicative of sulfate-reducers as well as the mcrA gene of methanogens were detected in the Benguela upwelling and Black Sea sediments. Overall, the high organic carbon content of the sediments goes along with high cell counts and high gene copy numbers, as well as an equal abundance of Bacteria and Archaea. eng
dc.description.sponsorship DFG/IODP/ODP/SCHI 535/7
dc.language.iso eng
dc.publisher Lausanne : Frontiers Research Foundation
dc.relation.ispartofseries Frontiers in Microbiology 3 (2012)
dc.rights CC BY-NC 3.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc/3.0/
dc.subject benguela upwelling eng
dc.subject black sea eng
dc.subject card-fish eng
dc.subject deep biosphere eng
dc.subject real-time pcr eng
dc.subject sediments eng
dc.subject subsurface eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Quantification of microbial communities in subsurface marine sediments of the Black Sea and off Namibia
dc.type Article
dc.type Text
dc.relation.issn 1664-302X
dc.relation.doi http://dx.doi.org/10.3389/fmicb.2012.00016
dc.bibliographicCitation.volume 3
dc.bibliographicCitation.firstPage 16
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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