An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/ macrophages

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dc.identifier.uri Beneke, Valerie ger Küster, Fennja ger Neehus, Anna-Lena ger Hesse, Christina ger Lopez-Rodriguez, Elena ger Rafiei Hashtchin, Anna ger Schott, Juliane Wilhelmine ger Walter, Dorothee ger Braun, Armin ger Wolkers, Willem F. ger Ackermann, Mania ger Lachmann, Nico ger 2019-04-23T07:10:15Z 2019-04-23T07:10:15Z 2018
dc.identifier.citation Beneke, V. et al.: An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. In: Scientific Reports (2018), Nr. 8, 16281. DOI: ger
dc.description.abstract Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform. ger
dc.language.iso eng ger
dc.publisher London : Macmillan Publishers Ltd
dc.relation.ispartofseries Scientific Reports (2018), Nr. 8 ger
dc.rights CC BY 4.0 Unported
dc.subject Macrophages eng
dc.subject Hematopoietic stem cell (HSC) eng
dc.subject Medicine eng
dc.subject Immune system eng
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/ macrophages eng
dc.type article ger
dc.type Text ger
dc.relation.issn 2045-2322
dc.relation.doi 10.1038/s41598-018-34524-2
dc.bibliographicCitation.firstPage 16281
dc.description.version publishedVersion ger
tib.accessRights frei zug�nglich

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