Tissue Engineered Skin Substitutes Created by Laser-Assisted Bioprinting Form Skin-Like Structures in the Dorsal Skin Fold Chamber in Mice

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dc.identifier.uri http://dx.doi.org/10.15488/4661
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4703
dc.contributor.author Michael, Stefanie
dc.contributor.author Sorg, Heiko
dc.contributor.author Peck, Claas-Tido
dc.contributor.author Koch, Lothar
dc.contributor.author Deiwick, Andrea
dc.contributor.author Chichkov, Boris
dc.contributor.author Vogt, Peter M.
dc.contributor.author Reimers, Kerstin
dc.date.accessioned 2019-03-28T12:29:34Z
dc.date.available 2019-03-28T12:29:34Z
dc.date.issued 2013
dc.identifier.citation Michael, S.; Sorg, H.; Peck, C.-T.; Koch, L.; Deiwick, A. et al.: Tissue Engineered Skin Substitutes Created by Laser-Assisted Bioprinting Form Skin-Like Structures in the Dorsal Skin Fold Chamber in Mice. In: PLoS ONE 8 (2013), Nr. 3, e57741. DOI: https://doi.org/10.1371/journal.pone.0057741
dc.description.abstract Tissue engineering plays an important role in the production of skin equivalents for the therapy of chronic and especially burn wounds. Actually, there exists no (cellularized) skin equivalent which might be able to satisfactorily mimic native skin. Here, we utilized a laser-assisted bioprinting (LaBP) technique to create a fully cellularized skin substitute. The unique feature of LaBP is the possibility to position different cell types in an exact three-dimensional (3D) spatial pattern. For the creation of the skin substitutes, we positioned fibroblasts and keratinocytes on top of a stabilizing matrix (Matriderm®). These skin constructs were subsequently tested in vivo, employing the dorsal skin fold chamber in nude mice. The transplants were placed into full-thickness skin wounds and were fully connected to the surrounding tissue when explanted after 11 days. The printed keratinocytes formed a multi-layered epidermis with beginning differentiation and stratum corneum. Proliferation of the keratinocytes was mainly detected in the suprabasal layers. In vitro controls, which were cultivated at the air-liquid-interface, also exhibited proliferative cells, but they were rather located in the whole epidermis. E-cadherin as a hint for adherens junctions and therefore tissue formation could be found in the epidermis in vivo as well as in vitro. In both conditions, the printed fibroblasts partly stayed on top of the underlying Matriderm® where they produced collagen, while part of them migrated into the Matriderm®. In the mice, some blood vessels could be found to grow from the wound bed and the wound edges in direction of the printed cells. In conclusion, we could show the successful 3D printing of a cell construct via LaBP and the subsequent tissue formation in vivo. These findings represent the prerequisite for the creation of a complex tissue like skin, consisting of different cell types in an intricate 3D pattern. eng
dc.language.iso eng
dc.publisher San Francisco, California, US : PLOS
dc.relation.ispartofseries PLoS ONE 8 (2013), Nr. 3
dc.rights CC BY 3.0
dc.rights.uri https://creativecommons.org/licenses/by/3.0/
dc.subject collagen eng
dc.subject uvomorulin eng
dc.subject angiogenesis eng
dc.subject animal experiment eng
dc.subject animal model eng
dc.subject animal tissue eng
dc.subject article eng
dc.subject bioprinting eng
dc.subject cell differentiation eng
dc.subject cell junction eng
dc.subject cell migration eng
dc.subject cell proliferation eng
dc.subject collagen synthesis eng
dc.subject controlled study eng
dc.subject epidermis eng
dc.subject extracellular matrix eng
dc.subject fibroblast eng
dc.subject in vitro study eng
dc.subject in vivo study eng
dc.subject keratinocyte eng
dc.subject laser assisted bioprinting eng
dc.subject mouse eng
dc.subject nonhuman eng
dc.subject skin graft eng
dc.subject skin injury eng
dc.subject skin transplantation eng
dc.subject skinfold eng
dc.subject stratum corneum eng
dc.subject tissue engineering eng
dc.subject Animals eng
dc.subject Biological Markers eng
dc.subject Bioprinting eng
dc.subject Burns eng
dc.subject Cadherins eng
dc.subject Cell Proliferation eng
dc.subject Cells, Cultured eng
dc.subject Collagen eng
dc.subject Elastin eng
dc.subject Fibroblasts eng
dc.subject Keratinocytes eng
dc.subject Lasers eng
dc.subject Mice eng
dc.subject Mice, Nude eng
dc.subject Neovascularization, Physiologic eng
dc.subject Skin eng
dc.subject Skin, Artificial eng
dc.subject Tissue Engineering eng
dc.subject Wound Healing eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Tissue Engineered Skin Substitutes Created by Laser-Assisted Bioprinting Form Skin-Like Structures in the Dorsal Skin Fold Chamber in Mice
dc.type article
dc.type Text
dc.relation.issn 1932-6203
dc.relation.doi https://doi.org/10.1371/journal.pone.0057741
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 8
dc.bibliographicCitation.firstPage e57741
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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