Genetic variation of temperature-regulated curd induction in cauliflower: elucidation of floral transition by genome-wide association mapping and gene expression analysis

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dc.identifier.uri http://dx.doi.org/10.15488/442
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/465
dc.contributor.author Matschegewski, Claudia
dc.contributor.author Zetzsche, Holger
dc.contributor.author Hasan, Yaser
dc.contributor.author Leibeguth, Lena
dc.contributor.author Briggs, William
dc.contributor.author Ordon, Frank
dc.contributor.author Uptmoor, Ralf
dc.date.accessioned 2016-08-29T08:04:53Z
dc.date.available 2016-08-29T08:04:53Z
dc.date.issued 2015-09-10
dc.identifier.citation Matschegewski, Claudia; Zetzsche, Holger; Hasan, Yaser; Leibeguth, Lena; Briggs, William et al.: Genetic variation of temperature-regulated curd induction in cauliflower: elucidation of floral transition by genome-wide association mapping and gene expression analysis. In: Frontiers in Plant Science 6 (2015), 720. DOI: http://dx.doi.org/10.3389/fpls.2015.00720
dc.description.abstract Cauliflower (Brassica oleracea var. botrytis) is a vernalization-responsive crop. High ambient temperatures delay harvest time. The elucidation of the genetic regulation of floral transition is highly interesting for a precise harvest scheduling and to ensure stable market supply. This study aims at genetic dissection of temperature-dependent curd induction in cauliflower by genome-wide association studies and gene expression analysis. To assess temperature dependent curd induction, two greenhouse trials under distinct temperature regimes were conducted on a diversity panel consisting of 111 cauliflower commercial parent lines, genotyped with 14,385 SNPs. Broad phenotypic variation and high heritability (0.93) were observed for temperature-related curd induction within the cauliflower population. GWA mapping identified a total of 18 QTL localized on chromosomes O1, O2, O3, O4, O6, O8, and O9 for curding time under two distinct temperature regimes. Among those, several QTL are localized within regions of promising candidate flowering genes. Inferring population structure and genetic relatedness among the diversity set assigned three main genetic clusters. Linkage disequilibrium (LD) patterns estimated global LD extent of r(2) = 0.06 and a maximum physical distance of 400 kb for genetic linkage. Transcriptional profiling of flowering genes FLOWERING LOCUS C (BoFLC) and VERNALIZATION 2 (BoVRN2) was performed, showing increased expression levels of BoVRN2 in genotypes with faster curding. However, functional relevance of BoVRN2 and BoFLC2 could not consistently be supported, which probably suggests to act facultative and/or might evidence for BoVRN2/BoFLC-independent mechanisms in temperature regulated floral transition in cauliflower. Genetic insights in temperature-regulated curd induction can underpin genetically informed phenology models and benefit molecular breeding strategies toward the development of thermo-tolerant cultivars. eng
dc.description.sponsorship BMBF/AgroCluster/0315542A
dc.language.iso eng
dc.publisher Lausanne : Frontiers Media Sa
dc.relation.ispartofseries Frontiers in Plant Science 6 (2015)
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject genome-wide association study (gwas) eng
dc.subject quantitative trait loci (qtl) eng
dc.subject transcriptional profiling eng
dc.subject single nucleotide polymorphism (snp) eng
dc.subject linkage disequilibrium (ld) eng
dc.subject vernalization eng
dc.subject cauliflower eng
dc.subject curd induction eng
dc.subject quantitative trait loci eng
dc.subject maize inbred lines eng
dc.subject flowering-time eng
dc.subject brassica-oleracea eng
dc.subject linkage disequilibrium eng
dc.subject structured populations eng
dc.subject sequence polymorphism eng
dc.subject qtl analysis eng
dc.subject vernalization eng
dc.subject arabidopsis eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Genetic variation of temperature-regulated curd induction in cauliflower: elucidation of floral transition by genome-wide association mapping and gene expression analysis
dc.type article
dc.type Text
dc.relation.issn 1664-462X
dc.relation.doi http://dx.doi.org/10.3389/fpls.2015.00720
dc.bibliographicCitation.volume 6
dc.bibliographicCitation.firstPage 720
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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