Transcriptome, carbohydrate, and phytohormone analysis of Petunia hybrida reveals a complex disturbance of plant functional integrity under mild chilling stress

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dc.identifier.uri http://dx.doi.org/10.15488/438
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/461
dc.contributor.author Bauerfeind, Martin Andreas
dc.contributor.author Winkelmann, Traud
dc.contributor.author Franken, Philipp
dc.contributor.author Druege, Uwe
dc.date.accessioned 2016-08-29T08:04:52Z
dc.date.available 2016-08-29T08:04:52Z
dc.date.issued 2015-07-28
dc.identifier.citation Bauerfeind, Martin Andreas; Winkelmann, Traud; Franken, Philipp; Druege, Uwe: Transcriptome, carbohydrate, and phytohormone analysis of Petunia hybrida reveals a complex disturbance of plant functional integrity under mild chilling stress. In: Frontiers in Plant Science 6 (2015), 583. DOI: http://dx.doi.org/10.3389/fpls.2015.00583
dc.description.abstract Cultivation of chilling-tolerant ornamental crops at lower temperature could reduce the energy demands of heated greenhouses. To provide a better understanding of how sub-optimal temperatures (12 degrees C vs. 16 degrees C) affect growth of the sensitive Petunia hybrida cultivar 'SweetSunshine Williams', the transcriptome, carbohydrate metabolism, and phytohormone homeostasis were monitored in aerial plant parts over 4 weeks by use of a microarray, enzymatic assays and GC-MS/MS. The data revealed three consecutive phases of chilling response. The first days were marked by a strong accumulation of sugars, particularly in source leaves, preferential up-regulation of genes in the same tissue and down-regulation of several genes in the shoot apex, especially those involved in the abiotic stress response. The midterm phase featured a partial normalization of carbohydrate levels and gene expression. After 3 weeks of chilling exposure, a new stabilized balance was established. Reduced hexose levels in the shoot apex, reduced ratios of sugar levels between the apex and source leaves and a higher apical sucrose/hexose ratio, associated with decreased activity and expression of cell wall invertase, indicate that prolonged chilling induced sugar accumulation in source leaves at the expense of reduced sugar transport to and reduced sucrose utilization in the shoot. This was associated with reduced levels of indole-3-acetic acid and abscisic acid in the apex and high numbers of differentially, particularly up-regulated genes, especially in the source leaves, including those regulating histones, ethylene action, transcription factors, and a jasmonate-ZIM-domain protein. Transcripts of one Jumonji C domain containing protein and one expansin accumulated in source leaves throughout the chilling period. The results reveal a dynamic and complex disturbance of plant function in response to mild chilling, opening new perspectives for the comparative analysis of differently tolerant cultivars. eng
dc.description.sponsorship BMBF/AgroCluster/WeGa - Horticulture Research Network
dc.description.sponsorship State of Brandenburg
dc.description.sponsorship Free State of Thuringia
dc.description.sponsorship Federal Republic of Germany
dc.language.iso eng
dc.publisher Lausanne : Frontiers Media Sa
dc.relation.ispartofseries Frontiers in Plant Science 6 (2015)
dc.rights CC BY 4.0
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject petunia eng
dc.subject cold eng
dc.subject carbohydrate metabolism eng
dc.subject sugars eng
dc.subject invertase eng
dc.subject gene expression eng
dc.subject microarray eng
dc.subject plant hormones eng
dc.subject freezing tolerance eng
dc.subject arabidopsis-thaliana eng
dc.subject abscisic-acid eng
dc.subject suboptimal temperature eng
dc.subject carbon metabolism eng
dc.subject cold-acclimation eng
dc.subject expression eng
dc.subject seedlings eng
dc.subject growth eng
dc.subject auxin eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Transcriptome, carbohydrate, and phytohormone analysis of Petunia hybrida reveals a complex disturbance of plant functional integrity under mild chilling stress
dc.type article
dc.type Text
dc.relation.issn 1664-462X
dc.relation.doi http://dx.doi.org/10.3389/fpls.2015.00583
dc.bibliographicCitation.volume 6
dc.bibliographicCitation.firstPage 583
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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