A combined method for correlative 3D imaging of biological samples from macro to nano scale

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dc.identifier.uri http://dx.doi.org/10.15488/4572
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4614
dc.contributor.author Kellner, Manuela
dc.contributor.author Heidrich, Marko
dc.contributor.author Lorbeer, Raoul-Amadeus
dc.contributor.author Antonopoulos, Georgios C.
dc.contributor.author Knudsen, Lars
dc.contributor.author Wrede, Christoph
dc.contributor.author Izykowski, Nicole
dc.contributor.author Grothausmann, Roman
dc.contributor.author Jonigk, Danny
dc.contributor.author Ochs, Matthias
dc.contributor.author Ripken, Tammo
dc.contributor.author Kühnel, Mark P.
dc.contributor.author Meyer, Heiko
dc.date.accessioned 2019-03-27T11:25:33Z
dc.date.available 2019-03-27T11:25:33Z
dc.date.issued 2016
dc.identifier.citation Kellner, M.; Heidrich, M.; Lorbeer, R.-A.; Antonopoulos, G.C.; Knudsen, L. et al.: A combined method for correlative 3D imaging of biological samples from macro to nano scale. In: Scientific Reports 6 (2016), 35606. DOI: https://doi.org/10.1038/srep35606
dc.description.abstract Correlative analysis requires examination of a specimen from macro to nano scale as well as applicability of analytical methods ranging from morphological to molecular. Accomplishing this with one and the same sample is laborious at best, due to deformation and biodegradation during measurements or intermediary preparation steps. Furthermore, data alignment using differing imaging techniques turns out to be a complex task, which considerably complicates the interconnection of results. We present correlative imaging of the accessory rat lung lobe by combining a modified Scanning Laser Optical Tomography (SLOT) setup with a specially developed sample preparation method (CRISTAL). CRISTAL is a resin-based embedding method that optically clears the specimen while allowing sectioning and preventing degradation. We applied and correlated SLOT with Multi Photon Microscopy, histological and immunofluorescence analysis as well as Transmission Electron Microscopy, all in the same sample. Thus, combining CRISTAL with SLOT enables the correlative utilization of a vast variety of imaging techniques. eng
dc.language.iso eng
dc.publisher London : Nature Publishing Group
dc.relation.ispartofseries Scientific Reports 6 (2016)
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject animal eng
dc.subject lung eng
dc.subject multimodal imaging eng
dc.subject pathology eng
dc.subject procedures eng
dc.subject rat eng
dc.subject three dimensional imaging eng
dc.subject tomography eng
dc.subject Animals eng
dc.subject Imaging, Three-Dimensional eng
dc.subject Lung eng
dc.subject Multimodal Imaging eng
dc.subject Pathology eng
dc.subject Rats eng
dc.subject Tomography eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 600 | Technik ger
dc.title A combined method for correlative 3D imaging of biological samples from macro to nano scale
dc.type article
dc.type Text
dc.relation.issn 2045-2322
dc.relation.doi https://doi.org/10.1038/srep35606
dc.bibliographicCitation.volume 6
dc.bibliographicCitation.firstPage 35606
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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