Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes

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dc.identifier.uri http://dx.doi.org/10.15488/437
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/460
dc.contributor.author Effendi, Yunus
dc.contributor.author Ferro, Noel
dc.contributor.author Labusch, Corinna
dc.contributor.author Geisler, Markus
dc.contributor.author Scherer, Günther F. E.
dc.date.accessioned 2016-08-29T08:04:52Z
dc.date.available 2016-08-29T08:04:52Z
dc.date.issued 2015-01
dc.identifier.citation Effendi, Yunus; Ferro, Noel; Labusch, Corinna; Geisler, Markus; Scherer, Guenther F. E.: Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes. In: Journal of Experimental Botany 66 (2015), Nr. 1, S. 403-418. DOI: http://dx.doi.org/10.1093/jxb/eru433
dc.description.abstract The function of the extracytoplasmic AUXIN-BINDING-PROTEIN1 (ABP1) is largely enigmatic. We complemented a homozygous T-DNA insertion null mutant of ABP1 in Arabidopsis thaliana Wassilewskia with three mutated and one wild-type (wt) ABP1 cDNA, all tagged C-terminally with a strepII-FLAG tag upstream the KDEL signal. Based on in silico modelling, the abp1 mutants were predicted to have altered geometries of the auxin binding pocket and calculated auxin binding energies lower than the wt. Phenotypes linked to auxin transport were compromised in these three complemented abp1 mutants. Red light effects, such as elongation of hypocotyls in constant red (R) and far-red (FR) light, in white light supplemented by FR light simulating shade, and inhibition of gravitropism by R or FR, were all compromised in the complemented lines. Using auxin-or light-induced expression of marker genes, we showed that auxininduced expression was delayed already after 10 min, and light-induced expression within 60 min, even though TIR1/AFB or phyB are thought to act as receptors relevant for gene expression regulation. The expression of marker genes in seedlings responding to both auxin and shade showed that for both stimuli regulation of marker gene expression was altered after 10-20 min in the wild type and phyB mutant. The rapidity of expression responses provides a framework for the mechanics of functional interaction of ABP1 and phyB to trigger interwoven signalling pathways. eng
dc.description.sponsorship DLR/50WB0627
dc.description.sponsorship DLR/50WB0933
dc.description.sponsorship DLR/50BW1333
dc.language.iso eng
dc.publisher Oxford : Oxford Univ. Press
dc.relation.ispartofseries Journal of Experimental Botany 66 (2015), Nr. 1
dc.rights CC BY 3.0
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject auxin-binding protein1 (abp1) eng
dc.subject abp1 mutants eng
dc.subject early auxin-regulated genes eng
dc.subject early light-regulated genes eng
dc.subject gravitropism eng
dc.subject phototropism eng
dc.subject phytochrome eng
dc.subject hypocotyl elongation eng
dc.subject shade avoidance eng
dc.subject arabidopsis thaliana eng
dc.subject shade avoidance-response eng
dc.subject arabidopsis-thaliana eng
dc.subject cell expansion eng
dc.subject signal-transduction eng
dc.subject negative regulator eng
dc.subject plant development eng
dc.subject gene-expression eng
dc.subject red-light eng
dc.subject transport eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes
dc.type article
dc.type Text
dc.relation.essn 1460-2431
dc.relation.issn 0022-0957
dc.relation.doi http://dx.doi.org/10.1093/jxb/eru433
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 66
dc.bibliographicCitation.firstPage 403
dc.bibliographicCitation.lastPage 418
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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