Low CO2 induces urea cycle intermediate accumulation in Arabidopsis thaliana

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dc.identifier.uri http://dx.doi.org/10.15488/4426
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4466
dc.contributor.author Blume, Christian
dc.contributor.author Ost, Julia
dc.contributor.author Mühlenbruch, Marco
dc.contributor.author Peterhänsel, Christoph
dc.contributor.author Laxa, Miriam
dc.date.accessioned 2019-01-31T10:02:01Z
dc.date.available 2019-01-31T10:02:01Z
dc.date.issued 2019
dc.identifier.citation Blume, C.; Ost, J.; Mühlenbruch, M.; Peterhänsel, C.; Laxa, M.: Low CO2 induces urea cycle intermediate accumulation in Arabidopsis thaliana. In: PLoS ONE 14 (2019), Nr. 1, e0210342. DOI: https://doi.org/10.1371/journal.pone.0210342
dc.description.abstract The non-proteinogenic amino acid ornithine links several stress response pathways. From a previous study we know that ornithine accumulates in response to low CO2. To investigate ornithine accumulation in plants, we shifted plants to either low CO2 or low light. Both conditions increased carbon limitation, but only low CO2 also increased the rate of photorespiration. Changes in metabolite profiles of light- and CO2-limited plants were quite similar. Several amino acids that are known markers of senescence accumulated strongly under both conditions. However, urea cycle intermediates respond differently between the two treatments. While the levels of both ornithine and citrulline were much higher in plants shifted to 100 ppm CO2 compared to those kept in 400 ppm CO2, their metabolite abundance did not significantly change in response to a light limitation. Furthermore, both ornithine and citrulline accumulation is independent from sugar starvation. Exogenous supplied sugar did not significantly change the accumulation of the two metabolites in low CO2-stressed plants, while the accumulation of other amino acids was reduced by about 50%. Gene expression measurements showed a reduction of the entire arginine biosynthetic pathway in response to low CO2. Genes in both proline biosynthesis and degradation were induced. Hence, proline did not accumulate in response to low CO2 like observed for many other stresses. We propose that excess of nitrogen re-fixed during photorespiration can be alternatively stored in ornithine and citrulline under low CO2 conditions. Furthermore, ornithine is converted to pyrroline-5-carboxylate by the action of δOAT. eng
dc.language.iso eng
dc.publisher San Francisco, CA : Public Library of Science (PLoS)
dc.relation.ispartofseries PLoS ONE 14 (2019), Nr. 1
dc.rights CC BY 4.0
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Arabidopsis thaliana eng
dc.subject CO2 eng
dc.subject Photosynthesis eng
dc.subject RuBisCO eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Low CO2 induces urea cycle intermediate accumulation in Arabidopsis thaliana
dc.type article
dc.type Text
dc.relation.issn 1932-6203
dc.relation.doi https://doi.org/10.1371/journal.pone.0210342
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 14
dc.bibliographicCitation.firstPage e0210342
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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