Enabling second harmonic generation as a contrast mechanism for optical projection tomography (OPT) and scanning laser optical tomography (SLOT)

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dc.identifier.uri http://dx.doi.org/10.15488/4194
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4228
dc.contributor.author Nolte, Lena
dc.contributor.author Antonopoulos, Georgios C.
dc.contributor.author Rämisch, Lisa
dc.contributor.author Heisterkamp, Alexander
dc.contributor.author Ripken, Tammo
dc.contributor.author Meyer, Heiko
dc.date.accessioned 2018-12-17T13:14:07Z
dc.date.available 2018-12-17T13:14:07Z
dc.date.issued 2018
dc.identifier.citation Nolte, L.; Antonopoulos, G.C.; Rämisch, L.; Heisterkamp, A.; Ripken, T.; Meyer, H.: Enabling second harmonic generation as a contrast mechanism for optical projection tomography (OPT) and scanning laser optical tomography (SLOT). In: Biomedical Optics Express 9 (2018), Nr. 6, S. 2627-2639. DOI: https://doi.org/10.1364/BOE.9.002627
dc.description.abstract Volumetric imaging of connective tissue provides insights into the structure of biological tissue. Second harmonic generation (SHG) microscopy has become a standard method to image collagen rich tissue like skin or cornea. Due to the non-centrosymmetric architecture, no additional label is needed and tissue can be visualized noninvasively. Thus, SHG microscopy enables the investigation of collagen associated diseases, providing high resolution images and a field of view of several hundreds of µm. However, the in toto visualization of larger samples is limited to the working distance of the objective and the integration time of the microscope setup, which can sum up to several hours and days. A faster imaging technique for samples in the mesoscopic range is scanning laser optical tomography (SLOT), which provides linear fluorescence, scattering and absorption as intrinsic contrast mechanisms. Due to the advantages of SHG and the reduced measurement time of SLOT, the integration of SHG in SLOT would be a great extension. This way SHG measurements could be performed faster on large samples, providing isotropic resolution and simultaneous acquisition of all other contrast mechanisms available, such as fluorescence and absorption. SLOT is based on the principle of computed tomography, which requires the rotation of the sample. The SHG signal, however, depends strongly on the sample orientation and the polarization of the laser, which results in SHG intensity fluctuation during sample rotation and prevents successful 3D reconstruction. In this paper we investigate the angular dependence of the SHG signal by simulation and experiment and found a way to eliminate reconstruction artifacts caused by this angular dependence in SHG-SLOT data. This way, it is now possible to visualize samples in the mesoscopic range using SHG-SLOT, with isotropic resolution and in correlation to other contrast mechanisms as absorption, fluorescence and scattering. eng
dc.language.iso eng
dc.publisher Washington, DC : Optical Society of America
dc.relation.ispartofseries Biomedical Optics Express 9 (2018), Nr. 6
dc.rights OSA Open Access License
dc.rights.uri https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.subject Biomedical signal processing eng
dc.subject Collagen eng
dc.subject Computerized tomography eng
dc.subject Fluorescence eng
dc.subject Musculoskeletal system eng
dc.subject Nonlinear optics eng
dc.subject Optical tomography eng
dc.subject Scanning eng
dc.subject Superconducting materials eng
dc.subject Tissue eng
dc.subject High resolution image eng
dc.subject Isotropic resolution eng
dc.subject Non-centrosymmetric eng
dc.subject Optical projection tomography eng
dc.subject Reconstruction artifacts eng
dc.subject Scattering and absorption eng
dc.subject Second harmonic generation microscopies (SHG) eng
dc.subject Simultaneous acquisition eng
dc.subject Harmonic generation eng
dc.subject.ddc 530 | Physik ger
dc.title Enabling second harmonic generation as a contrast mechanism for optical projection tomography (OPT) and scanning laser optical tomography (SLOT)
dc.type article
dc.type Text
dc.relation.issn 21567085
dc.relation.doi https://doi.org/10.1364/BOE.9.002627
dc.bibliographicCitation.issue 6
dc.bibliographicCitation.volume 9
dc.bibliographicCitation.firstPage 2627
dc.bibliographicCitation.lastPage 2639
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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