Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves

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dc.identifier.uri http://dx.doi.org/10.15488/4163
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4197
dc.contributor.author Brandt, Saskia
dc.contributor.author Fachinger, Sara
dc.contributor.author Tohge, Takayuki
dc.contributor.author Fernie, Alisdair R.
dc.contributor.author Braun, Hans-Peter
dc.contributor.author Hildebrandt, Tatjana M.
dc.date.accessioned 2018-12-14T13:58:55Z
dc.date.available 2018-12-14T13:58:55Z
dc.date.issued 2018
dc.identifier.citation Brandt, S.; Fachinger, S.; Tohge, T.; Fernie, A.R.; Braun, H.-P.; Hildebrandt, T.M.: Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. In: PLoS ONE 13 (2018), Nr. 8, e0202153. DOI: https://doi.org/10.1371/journal.pone.0202153
dc.description.abstract Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production. We investigated, whether the internal breakdown of glucosinolates, a major class of sulfur-containing secondary metabolites, might be an additional component of the carbohydrate starvation response in Arabidopsis thaliana (A. thaliana). The glucosinolate content of A. thaliana leaves was strongly reduced after seven days of darkness. We also detected a significant increase in the activity of myrosinase, the enzyme catalyzing the initial step in glucosinolate breakdown, coinciding with a strong induction of the main leaf myrosinase isoforms TGG1 and TGG2. In addition, nitrilase activity was increased suggesting a turnover via nitriles and carboxylic acids. Internal degradation of glucosinolates might also be involved in diurnal or developmental adaptations of the glucosinolate profile. We observed a diurnal rhythm for myrosinase activity in two-week-old plants. Furthermore, leaf myrosinase activity and protein abundance of TGG2 varied during plant development, whereas leaf protein abundance of TGG1 remained stable indicating regulation at the transcriptional as well as post-translational level. eng
dc.language.iso eng
dc.publisher San Francisco, CA : Public Library of Science
dc.relation.ispartofseries PLoS ONE 13 (2018), Nr. 8
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject carboxylic acid eng
dc.subject glucosinolate eng
dc.subject nitrilase eng
dc.subject nitrile eng
dc.subject TGG1 protein eng
dc.subject TGG2 protein eng
dc.subject thioglucosidase eng
dc.subject unclassified drug eng
dc.subject Arabidopsis thaliana eng
dc.subject Article eng
dc.subject circadian rhythm eng
dc.subject controlled study eng
dc.subject darkness eng
dc.subject nonhuman eng
dc.subject plant development eng
dc.subject plant leaf eng
dc.subject protein processing eng
dc.subject transcription regulation eng
dc.subject.ddc 580 | Pflanzen (Botanik) ger
dc.title Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves
dc.type Article
dc.type Text
dc.relation.issn 19326203
dc.relation.doi https://doi.org/10.1371/journal.pone.0202153
dc.bibliographicCitation.issue 8
dc.bibliographicCitation.volume 13
dc.bibliographicCitation.firstPage e0202153
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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