Analysis of the Aspergillus fumigatus Proteome Reveals Metabolic Changes and the Activation of the Pseurotin A Biosynthesis Gene Cluster in Response to Hypoxia

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dc.identifier.uri http://dx.doi.org/10.15488/4113
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4147
dc.contributor.author Vödisch, Martin ger
dc.contributor.author Scherlach, Kirstin ger
dc.contributor.author Winkler, Robert ger
dc.contributor.author Hertweck, Christian ger
dc.contributor.author Braun, Hans-Peter ger
dc.contributor.author Roth, Martin ger
dc.contributor.author Haas, Hubertus ger
dc.contributor.author Werner, Ernst R. ger
dc.contributor.author Brakhage, Axel A. ger
dc.contributor.author Kniemeyer, Olaf ger
dc.date.accessioned 2018-12-06T13:39:45Z
dc.date.available 2018-12-06T13:39:45Z
dc.date.issued 2011
dc.identifier.citation Vödisch, M.: Analysis of the Aspergillus fumigatus Proteome Reveals Metabolic Changes and the Activation of the Pseurotin A Biosynthesis Gene Cluster in Response to Hypoxia. In: Journal of Proteome Research 19 (2011), S. 2508-2524. DOI: https://doi.org/10.1021/pr1012812 ger
dc.description.abstract The mold Aspergillus fumigatus is the most important airborne fungal pathogen. Adaptation to hypoxia represents an important virulence attribute for A. fumigatus. Therefore, we aimed at obtaining a comprehensive overview about this process on the proteome level. To ensure highly reproducible growth conditions, an oxygen-controlled, glucose-limited chemostat cultivation was established. Two-dimensional gel electrophoresis analysis of mycelial and mitochondrial proteins as well as two-dimensional Blue Native/SDS-gel separation of mitochondrial membrane proteins led to the identification of 117 proteins with an altered abundance under hypoxic in comparison to normoxic conditions. Hypoxia induced an increased activity of glycolysis, the TCA-cycle, respiration, and amino acid metabolism. Consistently, the cellular contents in heme, iron, copper, and zinc increased. Furthermore, hypoxia induced biosynthesis of the secondary metabolite pseurotin A as demonstrated at proteomic, transcriptional, and metabolite levels. The observed and so far not reported stimulation of the biosynthesis of a secondary metabolite by oxygen depletion may also affect the survival of A. fumigatus in hypoxic niches of the human host. Among the proteins so far not implicated in hypoxia adaptation, an NO-detoxifying flavohemoprotein was one of the most highly up-regulated proteins which indicates a link between hypoxia and the generation of nitrosative stress in A. fumigatus. ger
dc.language.iso eng ger
dc.publisher Washington D.C. : American Chemical Society
dc.relation.ispartofseries Journal of Proteome Research 19 (2011) ger
dc.rights ACS AuthorChoice License ger
dc.rights.uri https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.subject Aspergillus fumigatus eng
dc.subject chemostat eng
dc.subject hypoxia eng
dc.subject mitochondria eng
dc.subject proteome analysis eng
dc.subject pseurotin A eng
dc.subject.ddc 540 | Chemie ger
dc.title Analysis of the Aspergillus fumigatus Proteome Reveals Metabolic Changes and the Activation of the Pseurotin A Biosynthesis Gene Cluster in Response to Hypoxia ger
dc.type article ger
dc.type Text ger
dc.relation.doi 10.1021/pr1012812
dc.description.version publishedVersion ger


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