Circadian Synthesis of Light-Harvesting-Chlorophyll-Proteins in Euglena gracilis Is under Translational Control

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dc.identifier.uri http://dx.doi.org/10.15488/3991
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/4025
dc.contributor.author Künne, A. ger
dc.contributor.author Pistorius, E. ger
dc.contributor.author Kloppstech, K. ger
dc.contributor.author de Groot, E. ger
dc.date.accessioned 2018-11-20T13:25:44Z
dc.date.available 2018-11-20T13:25:44Z
dc.date.issued 1998
dc.identifier.citation Künne, A.; Pistorius, E.; Kloppstech, K.; de Groot, E.: Circadian Synthesis of Light-Harvesting-Chlorophyll-Proteins in Euglena gracilis Is under Translational Control. In: Zeitschrift für Naturforschung C 53 (1998), S. 1017-1026. DOI: https://doi.org/10.1515/znc-1998-11-1213 ger
dc.description.abstract Two proteins with apparent molecular masses of 17 and 24 kD that are synthesized in a circadian manner in the phytoflagellate Euglena gracilis, were recognized as proteins belong­ing to the family of light-harvesting-chlorophyll-proteins (LHCPs) of class I (17 kD) and of class II (24 kD). Identification was achieved by N-terminal sequencing of the proteins isolated from two-dimensional polyacrylamide gels and by detection with an anti-LHCP II se­rum. While it was found that the total amount of LHCPs remains almost constant, when Euglena is grown under diurnal conditions (12 h light and 12 h dark), we could show that the amount of newly synthesized 17 and 24 kD proteins varies about 20-fold with a maximum of synthesis in the light phase. In contrast, the analysis of the mRNA levels at different times revealed only minor differences in the stationary concentration of the LHCP specific mRNA, indicating that the control of LHCP synthesis is at the translational level. Principally, the same finding was obtained using inhibitors of transcription. Thus, it is concluded that the expression of LHCPs in Euglena gracilis in contrast to that of higher plants is primarily regulated at the translational level. ger
dc.language.iso eng ger
dc.publisher Berlin, Boston : De Gruyter
dc.relation.ispartofseries Zeitschrift für Naturforschung C 53 (1998) ger
dc.rights CC BY-NC-ND 3.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject Euglena gracilis eng
dc.subject Circadian Rhythm eng
dc.subject Translational Control eng
dc.subject Protein Synthesis eng
dc.subject Light-Harvesting-Chlorophyll-Proteins eng
dc.subject.ddc 540 | Chemie ger
dc.title Circadian Synthesis of Light-Harvesting-Chlorophyll-Proteins in Euglena gracilis Is under Translational Control ger
dc.type article ger
dc.type Text ger
dc.relation.essn 1865-7125
dc.relation.issn 0939-5075
dc.relation.doi 10.1515/znc-1998-11-1213
dc.bibliographicCitation.firstPage 1017
dc.bibliographicCitation.lastPage 1026
dc.description.version publishedVersion ger
tib.accessRights frei zug�nglich


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