In-situ microscopy and 2D fluorescence spectroscopy as online methods for monitoring CHO cells during cultivation

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dc.identifier.uri http://dx.doi.org/10.15488/3891
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/3925
dc.contributor.author Bonk, Sophia ger
dc.contributor.author Sandor, Marko ger
dc.contributor.author Rüdinger, Ferdinand ger
dc.contributor.author Tscheschke, Bernd ger
dc.contributor.author Prediger, Andreas ger
dc.contributor.author Babitzky, Alexander ger
dc.contributor.author Solle, Dørte ger
dc.contributor.author Beutel, Sascha ger
dc.contributor.author Scheper, Thomas ger
dc.date.accessioned 2018-10-26T13:24:12Z
dc.date.available 2018-10-26T13:24:12Z
dc.date.issued 2011
dc.identifier.citation Bonk, S.: In-situ microscopy and 2D fluorescence spectroscopy as online methods for monitoring CHO cells during cultivation. In: BMC Proceedings 5 (2011), P76. DOI: https://doi.org/10.1186/1753-6561-5-S8-P76 ger
dc.description.abstract Typical methods for monitoring cultivation processes are offline analyses like cell counting, measurement of various substrates and products (e. g. glucose or lactate) as well as the online monitoring of several physical process parameters (temperature, pH-value or the concentration of dissolved oxygen). To improve cell cultivations detailed information about important analytes should be available online. Therefore new monitoring methods need to be established, preferably as in-situ methods to minimize the risk of contamination. Two different in-situ online-methods were used to monitor cultivations: In-situ microscopy and 2D fluorescence spectroscopy. Therefore CHO-K1 cells (provided by University of Bielefeld) were cultivated in a complex culture medium (TC 42, TeutoCell, Bielefeld, Germany) using a 2.5 L stainless steel reactor with a work volume of 2 L. A total of three cultivation runs were conducted. ger
dc.language.iso eng ger
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartof BMC Proceedings 5 (2011) ger
dc.rights CC BY 2.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject Cell Density eng
dc.subject Calibration Model eng
dc.subject Cultivation Process eng
dc.subject Glutamate Concentration eng
dc.subject Online Monitoring eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title In-situ microscopy and 2D fluorescence spectroscopy as online methods for monitoring CHO cells during cultivation eng
dc.type conferenceObject ger
dc.type Text ger
dc.description.version publishedVersion ger
tib.accessRights frei zug�nglich


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