Approaches for automized expansion and differentiation of human MSC in specialized bioreactors

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dc.identifier.uri http://dx.doi.org/10.15488/3888
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/3922
dc.contributor.author Neumann, Anne ger
dc.contributor.author Lavrentieva, Antonina ger
dc.contributor.author Egger, Dominik ger
dc.contributor.author Hatlapatka, Tim ger
dc.contributor.author Kasper, Cornelia ger
dc.date.accessioned 2018-10-26T12:07:40Z
dc.date.available 2018-10-26T12:07:40Z
dc.date.issued 2013
dc.identifier.citation Neumann, A. et al.: Approaches for automized expansion and differentiation of human MSC in specialized bioreactors. In: BMC Proceedings 7 (2013), P47. DOI: https://doi.org/10.1186/1753-6561-7-S6-P47 ger
dc.description.abstract A main challenge in cell therapies and other tissue regeneration approaches is to produce a therapeutically significant cell number. For expansion of mesenchymal stem cells (MSC) the cultivation on 2D plastic surfaces is still the conventional procedure, even though the culture conditions differ significantly from the 3D environment in vivo. Additionally, static amplification of MSC is a labour-intensive procedure. We therefore used a specialized rotating bed bioreactor in order to maximize ex vivo expansion of MSC. MSC were isolated from umbilical cord (UC) by explant method approach under xeno-free conditions. UC-MSC were thereafter expanded under dynamic conditions in a novel rotating bed bioreactor. The bioreactor system was designed to enable integration of sensors for online monitoring of various parameters (e.g. pH, pO2, pCO2) and hence, allow ensured cultivation under well controlled and reproducible conditions. Beside cell expansion, directed differentiation of MSC was also achieved in bioreactors. MSC lack the ability to grow in 3D direction and build functional tissue in vitro. Thus, it is necessary to seed and culture MSC on 3D matrices to obtain functional implants. For guided differentiation towards the osteogenic lineage, MSC were cultivated on ceramic porous matrices under dynamic conditions. Custom-made miniaturized perfusion bioreactors for parallel testing were designed and optimized for that purpose. ger
dc.language.iso eng ger
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartof BMC Proceedings 7 (2013) ger
dc.rights CC BY 2.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject Mesenchymal Stem Cell eng
dc.subject Osteogenic Differentiation eng
dc.subject Dynamic Cultivation eng
dc.subject Osteogenic Lineage eng
dc.subject Perfusion Bioreactor eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Approaches for automized expansion and differentiation of human MSC in specialized bioreactors eng
dc.type conferenceObject ger
dc.type Text ger
dc.description.version publishedVersion ger
tib.accessRights frei zug�nglich


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