Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway

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dc.identifier.uri Remelli, William Guerrieri, Nicoletta Klodmann, Jennifer Papenbrock, Jutta Pagani, Silvia Forlani, Fabio 2015-07-31T11:17:09Z 2015-07-31T11:17:09Z 2012
dc.identifier.citation Remelli, William; Guerrieri, Nicoletta; Klodmann, Jennifer; Papenbrock, Jutta; Pagani, Silvia; Forlani, Fabio: Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway. In: PLoS ONE 7 (2012), Nr. 9. DOI:
dc.description.abstract The phenotypic features of the Azotobacter vinelandii RhdA mutant MV474 (in which the rhdA gene was deleted) indicated that defects in antioxidant systems in this organism were related to the expression of the tandem-domain rhodanese RhdA. In this work, further insights on the effects of the oxidative imbalance generated by the absence of RhdA (e.g. increased levels of lipid hydroperoxides) are provided. Starting from the evidence that glutathione was depleted in MV474, and using both in silico and in vitro approaches, here we studied the interaction of wild-type RhdA and Cys(230) Ala site-directed RhdA mutant with glutathione species. We found that RhdA was able to bind in vitro reduced glutathione (GSH) and that RhdA-Cys(230) residue was mandatory for the complex formation. RhdA catalyzed glutathione-disulfide formation in the presence of a system generating the glutathione thiyl radical (GS(.), an oxidized form of GSH), thereby facilitating GSH regeneration. This reaction was negligible when the Cys230 Ala RhdA mutant was used. The efficiency of RhdA as catalyst in GS(.)-scavenging activity is discussed on the basis of the measured parameters of both interaction with glutathione species and kinetic studies. eng
dc.description.sponsorship Vigoni project/0815171
dc.description.sponsorship Deutscher Akademischer Austausch Dienst
dc.description.sponsorship Universita` degli Studi di Milano/Fondo interno ricerca scientifica e tecnologica
dc.language.iso eng eng
dc.publisher San Francisco : Public Library of Science
dc.relation.ispartofseries PLoS ONE 7 (2012), Nr. 9
dc.rights CC BY 3.0 Unported
dc.subject RhdA eng
dc.subject Rhodanese-like proteins eng
dc.subject Azotobacter vinelandii eng
dc.subject Glutathione eng
dc.subject Oxidative stress eng
dc.subject Thiyl radicals eng
dc.subject Settore BIO/10 - Biochimica eng
dc.subject RhdA ger
dc.subject Rhodanese ger
dc.subject Azotobacter vinelandii ger
dc.subject Glutathion eng
dc.subject Oxidativer Stress ger
dc.subject Glutathion ger
dc.subject.classification Thiosulfat-Sulfurtransferase ger
dc.subject.classification Azotobacter vinelandii ger
dc.subject.classification Glutathion ger
dc.subject.classification Oxidativer Stress ger
dc.subject.ddc 580 | Pflanzen (Botanik)
dc.title Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway eng
dc.type Article
dc.type Text
dc.relation.issn 1932-6203
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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