dc.identifier.uri |
http://dx.doi.org/10.15488/3649 |
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dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/3681 |
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dc.contributor.author |
Trenti, Francesco
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dc.date.accessioned |
2018-08-27T12:10:18Z |
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dc.date.available |
2018-08-27T12:10:18Z |
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dc.date.issued |
2018 |
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dc.identifier.citation |
Trenti, Francesco: The biosynthesis of phyllostictine A and Sch-642305 from Phyllosticta cirsii and Phomopsis CMU-LMA. Hannover : Gottfried Wilhelm Leibniz Universität, Diss., 2018, xi, 186 S. DOI: https://doi.org/10.15488/3649 |
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dc.description.abstract |
The presented work focuses on the biosynthesis of fungal natural products. Structural analysis of phyllostictine A revealed the literature structure to be erroneous and led to the revised bicyclic 3-methylene tetramic acid. In particular, feeding 13 C-labeled precursors showed a haphazard incorporation into and set the basis for the correct structure elucidation. The Biosynthetic Gene Cluster (BGC) was identified by total genome sequencing of the producer (P. cirsii ) and in silico analyis. Targeted knock out experiments confirmed the BGC to be involved in phyllostictine A biosynthesis and produced an intermediate. In the attempt to define each biosynthetic step that takes part in Sch-642305 biosynthesis, two intermediates were observed after targeted knock out of a cytochrome P450 cytochrome and a flavoprotein oxidase, respectively. Genome assembly and in silico analysis showed over 150 BGC for this strain. The sch-related BGC was defined by rational survey and homology comparison with other producing organisms, such as Penicillium verrucosum and Penicillium brefeldianum, producers of Sch-642305 and Brefeldin A respectively. Although, other genes of Sch-642305 cluster were successfully disrupted, no other intermediate was observed. |
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dc.language.iso |
eng |
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dc.publisher |
Hannover : Institutionelles Repositorium der Leibniz Universität Hannover |
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dc.rights |
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. |
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dc.subject |
natural products |
eng |
dc.subject |
biosynthesis |
eng |
dc.subject |
fungi |
eng |
dc.subject |
Pilze |
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dc.subject |
Poliketide |
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dc.subject |
Biosynthese |
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dc.subject |
Sekundärmetabolite |
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dc.subject.ddc |
540 | Chemie
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dc.title |
The biosynthesis of phyllostictine A and Sch-642305 from Phyllosticta cirsii and Phomopsis CMU-LMA |
eng |
dc.type |
DoctoralThesis |
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dc.type |
Text |
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dcterms.extent |
xi, 186 S. |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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