The mycorrhiza-dependent defensin MtDefMd1 of Medicago truncatula acts during the late restructuring stages of arbusculecontaining cells

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dc.identifier.uri http://dx.doi.org/10.15488/3393
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/3423
dc.contributor.author Uhe, Marian
dc.contributor.author Hogekamp, Claudia
dc.contributor.author Hartmann, Rico M.
dc.contributor.author Hohnjec, Natalija
dc.contributor.author Küster, Helge
dc.date.accessioned 2018-05-23T11:04:02Z
dc.date.available 2018-05-23T11:04:02Z
dc.date.issued 2018
dc.identifier.citation Uhe, M.; Hogekamp, C.; Hartmann, R.M.; Hohnjec, N.; Küster, H.: The mycorrhiza-dependent defensin MtDefMd1 of Medicago truncatula acts during the late restructuring stages of arbusculecontaining cells. In: PLoS ONE 13 (2018), Nr. 1, e0191841. DOI: https://doi.org/10.1371/journal.pone.0191841
dc.description.abstract Different symbiotic and pathogenic plant-microbe interactions involve the production of cysteine- rich antimicrobial defensins. In Medicago truncatula, the expression of four MtDefMd genes, encoding arbuscular mycorrhiza-dependent defensins containing an N-terminal signal peptide and exhibiting some differences to non-symbiotic defensins, raised over the time of fungal colonization. Whereas the MtDefMd1 and MtDefMd2 promoters were inactive in cells containing young arbuscules, cells with fully developed arbuscules displayed different levels of promoter activities, indicating an up-regulation towards later stages of arbuscule formation. MtDefMd1 and MtDefMd2 expression was absent or strongly down-regulated in mycorrhized ram1-1 and pt4-2 mutants, known for defects in arbuscule branching or premature arbuscule degeneration, respectively. A ~97% knock-down of MtDefMd1/MtDefMd2 expression did not significantly affect arbuscule size. Although overexpression of MtDefMd1 in arbuscule-containing cells led to an up-regulation of MtRam1, encoding a key transcriptional regulator of arbuscule formation, no morphological changes were evident. Co-localization of an MtDefMd1-mGFP6 fusion with additional, subcellular markers revealed that this defensin is associated with arbuscules in later stages of their life-cycle. MtDefMd1-mGFP6 was detected in cells with older arbuscules about to collapse, and ultimately in vacuolar compartments. Comparisons with mycorrhized roots expressing a tonoplast marker indicated that MtDefMd1 acts during late restructuring processes of arbuscule-containing cells, upon their transition into a post-symbiotic state. © 2018 Uhe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. eng
dc.language.iso eng
dc.publisher San Francisco, CA : Public Library of Science
dc.relation.ispartofseries PLoS ONE 13 (2018), Nr. 1
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject barrel medic eng
dc.subject controlled study eng
dc.subject down regulation eng
dc.subject fungal gene eng
dc.subject gene expression eng
dc.subject gene knockdown eng
dc.subject gene location eng
dc.subject gene overexpression eng
dc.subject genetic transcription eng
dc.subject life cycle stage eng
dc.subject MtDefMd1 gene eng
dc.subject MtDefMd2 gene eng
dc.subject mutant eng
dc.subject mycorrhiza eng
dc.subject nonhuman eng
dc.subject promoter region eng
dc.subject upregulation eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title The mycorrhiza-dependent defensin MtDefMd1 of Medicago truncatula acts during the late restructuring stages of arbusculecontaining cells eng
dc.type article
dc.type Text
dc.relation.issn 1932-6203
dc.relation.doi https://doi.org/10.1371/journal.pone.0191841
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 13
dc.bibliographicCitation.firstPage e0191841
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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