Positive in vitro wound healing effects of functional inclusion bodies of a lipoxygenase from the Mexican axolotl

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dc.identifier.uri http://dx.doi.org/10.15488/3376
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/3406
dc.contributor.author Stamm, Anne
dc.contributor.author Straub, Sarah
dc.contributor.author Vogt, Peter
dc.contributor.author Scheper, Thomas
dc.contributor.author Pepelanova, Iliyana
dc.date.accessioned 2018-05-23T08:43:21Z
dc.date.available 2018-05-23T08:43:21Z
dc.date.issued 2018
dc.identifier.citation Stamm, A.; Straub, S.; Vogt, P.; Scheper, T.; Pepelanova, I.: Positive in vitro wound healing effects of functional inclusion bodies of a lipoxygenase from the Mexican axolotl. In: Microbial Cell Factories 17 (2018), Nr. 1, 57. DOI: https://doi.org/10.1186/s12934-018-0904-0
dc.description.abstract Background: AmbLOXe is a lipoxygenase, which is up-regulated during limb-redevelopment in the Mexican axolotl, Ambystoma mexicanum, an animal with remarkable regeneration capacity. Previous studies have shown that mammalian cells transformed with the gene of this epidermal lipoxygenase display faster migration and wound closure rate during in vitro wound healing experiments. Results: In this study, the gene of AmbLOXe was codon-optimized for expression in Escherichia coli and was produced in the insoluble fraction as protein aggregates. These inclusion bodies or nanopills were shown to be reservoirs containing functional protein during in vitro wound healing assays. For this purpose, functional inclusion bodies were used to coat cell culture surfaces prior cell seeding or were added directly to the medium after cells reached confluence. In both scenarios, AmbLOXe inclusion bodies led to faster migration rate and wound closure, in comparison to controls containing either no AmbLOXe or GFP inclusion bodies. Conclusions: Our results demonstrate that AmbLOXe inclusion bodies are functional and may serve as stable reservoirs of this enzyme. Nevertheless, further studies with soluble enzyme are also necessary in order to start elucidating the exact molecular substrates of AmbLOXe and the biochemical pathways involved in the wound healing effect. © 2018 The Author(s). eng
dc.language.iso eng
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartofseries Microbial Cell Factories 17 (2018), Nr. 1
dc.rights CC BY 4.0
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Active inclusion bodies eng
dc.subject AmbLOXe eng
dc.subject Axolotl (Ambystoma mexicanum) eng
dc.subject In vitro wound healing assay eng
dc.subject Lipoxygenase eng
dc.subject Nanopills eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Positive in vitro wound healing effects of functional inclusion bodies of a lipoxygenase from the Mexican axolotl
dc.type article
dc.type Text
dc.relation.issn 1475-2859
dc.relation.doi https://doi.org/10.1186/s12934-018-0904-0
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 17
dc.bibliographicCitation.firstPage 57
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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