Long-term delivery of brain-derived neurotrophic factor (BDNF) from nanoporous silica nanoparticles improves the survival of spiral ganglion neurons in vitro

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dc.identifier.uri http://dx.doi.org/10.15488/3355
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/3385
dc.contributor.author Schmidt, Nadeschda
dc.contributor.author Schulze, Jennifer
dc.contributor.author Warwas, Dawid P.
dc.contributor.author Ehlert, Nina
dc.contributor.author Lenarz, Thomas
dc.contributor.author Warnecke, Athanasia
dc.contributor.author Behrens, Peter
dc.date.accessioned 2018-05-23T06:37:56Z
dc.date.available 2018-05-23T06:37:56Z
dc.date.issued 2018
dc.identifier.citation Schmidt, N.; Schulze, J.; Warwas, D.P.; Ehlert, N.; Lenarz, T. et al.: Long-term delivery of brain-derived neurotrophic factor (BDNF) from nanoporous silica nanoparticles improves the survival of spiral ganglion neurons in vitro. In: PLoS ONE 13 (2018), Nr. 3, e0194778. DOI: https://doi.org/10.1371/journal.pone.0194778
dc.description.abstract Sensorineural hearing loss (SNHL) can be overcome by electrical stimulation of spiral ganglion neurons (SGNs) via a cochlear implant (CI). Restricted CI performance results from the spatial gap between the SGNs and the electrode, but the efficacy of CI is also limited by the degeneration of SGNs as one consequence of SHNL. In the healthy cochlea, the survival of SGNs is assured by endogenous neurotrophic support. Several applications of exogenous neurotrophic supply have been shown to reduce SGN degeneration in vitro and in vivo. In the present study, nanoporous silica nanoparticles (NPSNPs), with an approximate diameter of <100 nm, were loaded with the brain-derived neurotrophic factor (BDNF) to test their efficacy as long-term delivery system for neurotrophins. The neurotrophic factor was released constantly from the NPSNPs over a release period of 80 days when the surface of the nanoparticles had been modified with amino groups. Cell culture investigations with NIH3T3 fibroblasts attest a good general cytocompatibility of the NPSNPs. In vitro experiments with SGNs indicate a significantly higher survival rate of SGNs in cell cultures that contained BDNF-loaded nanoparticles compared to the control culture with unloaded NPSNPs (p<0.001). Importantly, also the amounts of BDNF released up to a time period of 39 days increased the survival rate of SGNs. Thus, NPSNPs carrying BDNF are suitable for the treatment of inner ear disease and for the protection and the support of SGNs. Their nanoscale nature and the fact that a direct contact of the nanoparticles and the SGNs is not necessary for neuroprotective effects, should allow for the facile preparation of nanocomposites, e.g., with biocompatible polymers, to install coatings on implants for the realization of implant-based growth factor delivery systems. © 2018 Schmidt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. eng
dc.language.iso eng
dc.publisher San Francisco, CA : Public Library of Science
dc.relation.ispartofseries PLoS ONE 13 (2018), Nr. 3
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject brain-derived neurotrophic factor eng
dc.subject BDNF eng
dc.subject Sensorineural hearing loss (SNHL) eng
dc.subject spiral ganglion neurons (SGNs) eng
dc.subject cochlear implant (CI) eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Long-term delivery of brain-derived neurotrophic factor (BDNF) from nanoporous silica nanoparticles improves the survival of spiral ganglion neurons in vitro
dc.type article
dc.type Text
dc.relation.issn 1932-6203
dc.relation.doi https://doi.org/10.1371/journal.pone.0194778
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 13
dc.bibliographicCitation.firstPage e0194778
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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