Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules

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dc.identifier.uri http://dx.doi.org/10.15488/283
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/305
dc.contributor.author Limpens, Erik
dc.contributor.author Moling, Sjef
dc.contributor.author Hooiveld, Guido
dc.contributor.author Pereira, Patricia A.
dc.contributor.author Bisseling, Ton
dc.contributor.author Becker, Joerg D.
dc.contributor.author Kuester, Helge
dc.date.accessioned 2016-06-13T14:56:06Z
dc.date.available 2016-06-13T14:56:06Z
dc.date.issued 2013-05-29
dc.identifier.citation Limpens, Erik; Moling, Sjef; Hooiveld, Guido; Pereira, Patricia A.; Bisseling, Ton; et. al.: Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules. In: PloS ONE 8 (2013), Nr. 5, e64377. DOI: http://dx.doi.org/10.1371/journal.pone.0064377
dc.description.abstract Legumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ''. This digital "in situ'' offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies. eng
dc.description.sponsorship Dutch Organization for Scientific Research/NWO 3184319448
dc.description.sponsorship Fundacao para a Ciencia e a Tecnologia/PTDC/AGR-GPL/70592/2006
dc.language.iso eng
dc.publisher San Francisco : Public Library Science
dc.relation.ispartofseries PLoS ONE 8 (2013), Nr. 5
dc.rights CC BY 4.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject subtractive hybridization approach eng
dc.subject nitrogen-fixing symbiosomes eng
dc.subject gene-expression eng
dc.subject sinorhizobium-meliloti eng
dc.subject rhizobium-meliloti eng
dc.subject lotus-japonicus eng
dc.subject n-2-fixing symbiosomes eng
dc.subject arbuscular mycorrhiza eng
dc.subject bacteroid development eng
dc.subject arabidopsis-thaliana eng
dc.subject.ddc 580 | Pflanzen (Botanik) ger
dc.title Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules eng
dc.type article
dc.type Text
dc.relation.essn 1932-6203
dc.relation.doi http://dx.doi.org/10.1371/journal.pone.0064377
dc.bibliographicCitation.issue 5
dc.bibliographicCitation.volume 8
dc.bibliographicCitation.firstPage e64377
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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