Zusammenfassung: | |
In this study we investigate the potential of femtosecond laser generated micrometer sized spike structures as functional surfaces for selective cell controlling. The spike dimensions as well as the average spike to spike distance can be easily tuned by varying the process parameters. Moreover, negative replications in soft materials such as silicone elastomer can be produced. This allows tailoring of wetting properties of the spike structures and their negative replicas representing a reduced surface contact area. Furthermore, we investigated material effects on cellular behavior. By comparing human fibroblasts and SH-SY5Y neuroblastoma cells we found that the influence of the material was cell specific. The cells not only changed their morphology, but also the cell growth was affected. Whereas, neuroblastoma cells proliferated at the same rate on the spike structures as on the control surfaces, the proliferation of fibroblasts was reduced by the spike structures. These effects can result from the cell specific adhesion patterns as shown in this work. These findings show a possibility to design defined surface microstructures, which could control cellular behavior in a cell specific manner.
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Lizenzbestimmungen: | Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2010 |
Schlagwörter (englisch): | biomedicine, cellular adhesion, femtosecond laser, microstructures, surface topography, water contact angle, biomedicine, Cellular adhesion, Cellular behaviors, femtosecond laser, Functional surfaces, Human fibroblast, Material effect, Neuroblastoma cells, Process parameters, Selective control, Silicone elastomers, Soft material, Surface contact, Surface microstructures, water contact angle, Wetting property, Adhesion, Cell culture, Contact angle, Fibroblasts, Growth kinetics, Microstructure, Pulsed laser applications, Silicones, Surface topography, Ultrashort pulses, Surfaces, biomaterial, elastomer, nanomaterial, silicon, article, cell adhesion, cell proliferation, cellular, subcellular and molecular biological phenomena and functions, chemistry, cytology, DNA damage, fibroblast, human, laser, metabolism, mutagen testing, neuroblastoma, surface property, time, tumor cell line, ultrastructure, Biocompatible Materials, Cell Adhesion, Cell Line, Tumor, Cell Physiological Processes, Cell Proliferation, DNA Damage, Elastomers, Fibroblasts, Humans, Lasers, Mutagenicity Tests, Nanostructures, Neuroblastoma, Silicon, Surface Properties, Time Factors |
Fachliche Zuordnung (DDC): | 610 | Medizin, Gesundheit |
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