Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus

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dc.identifier.uri http://dx.doi.org/10.15488/272
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/294
dc.contributor.author Eisenberg, Tobias
dc.contributor.author Nicklas, Werner
dc.contributor.author Mauder, Norman
dc.contributor.author Rau, Joerg
dc.contributor.author Contzen, Matthias
dc.contributor.author Semmler, Torsten
dc.contributor.author Hofmann, Nicola
dc.contributor.author Aledelbi, Khayrieh
dc.contributor.author Ewers, Christa
dc.date.accessioned 2016-06-13T14:30:48Z
dc.date.available 2016-06-13T14:30:48Z
dc.date.issued 2015-08-07
dc.identifier.citation Eisenberg, Tobias; Nicklas, Werner; Mauder, Norman; Rau, Joerg; Contzen, Matthias; Semmler, Torsten; Hofmann, Nicola; Aledelbi, Khayrieh; Ewers, Christa: Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus. In: PloS ONE 10 (2015), Nr. 8, e0134312. DOI: http://dx.doi.org/10.1371/journal.pone.0134312
dc.description.abstract The genus Streptobacillus (S.) remained monotypic for almost 90 years until two new species were recently described. The type species, S. moniliformis, is one of the two etiological agents of rat bite fever, an under-diagnosed, worldwide occurring zoonosis. In a polyphasic approach field isolates and reference strains of S. moniliformis, S. hongkongensis, S. felis as well as divergent isolates were characterized by comparison of molecular data (n = 29) and from the majority also by their physiological as well as proteomic properties (n = 22). Based on growth-independent physiological profiling using VITEK2-compact, API ZYM and the Micronaut system fastidious growth-related difficulties could be overcome and streptobacilli could definitively be typed despite generally few differences. While differing in their isolation sites and dates, S. moniliformis isolates were found to possess almost identical spectra in matrix-assisted laser desorption ionization-time of flight mass spectrometry and Fourier transform infrared spectroscopy. Spectroscopic methods facilitated differentiation of S. moniliformis, S. hongkongensis and S. felis as well as one divergent isolate. Sequencing of 16S rRNA gene as well as functional genes groEL, recA and gyrB revealed only little intraspecific variability, but generally proved suitable for interspecies discrimination between all three taxa and two groups of divergent isolates. eng
dc.description.sponsorship Hessian Ministry for the Environment, Climate Change, Agriculture and Consumer Protection (HMUKLV)
dc.description.sponsorship Hessian Ministry for the Environment, Climate Change, Agriculture and Consumer Protection (HMUKLV)
dc.language.iso eng
dc.publisher San Francisco : Public Library Science
dc.relation.ispartofseries PLoS ONE 10 (2015), Nr. 8
dc.rights CC BY 4.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject rat-bite fever eng
dc.subject polymerase-chain-reaction eng
dc.subject complete genome sequence eng
dc.subject hongkongensis sp nov. eng
dc.subject fusobacterium-nucleatum eng
dc.subject infrared-spectroscopy eng
dc.subject phylogenetic analysis eng
dc.subject fourier-transform eng
dc.subject ribosomal-rna eng
dc.subject moniliformis eng
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus
dc.type article
dc.type Text
dc.relation.essn 1932-6203
dc.relation.doi http://dx.doi.org/10.1371/journal.pone.0134312
dc.bibliographicCitation.issue 8
dc.bibliographicCitation.volume 10
dc.bibliographicCitation.firstPage e0134312
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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