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dc.identifier.uri Peters, Katrin Belt, Katharina Braun, Hans-Peter 2015-07-27T16:04:13Z 2015-07-27T16:04:13Z 2013-06-04
dc.identifier.citation Peters, Katrin; Belt, Katharina; Braun, Hans-Peter: 3D Gel Map of Arabidopsis Complex I. In: Frontiers in Plant Science 4 (2013), 153. DOI:
dc.description.abstract Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from Arabidopsis thaliana cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that Arabidopsis complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap is presented for promoting future complex I research in Arabidopsis thaliana. eng
dc.description.sponsorship DFG
dc.language.iso eng eng
dc.publisher Lausanne : Frontiers Research Foundation
dc.rights CC BY 3.0 Unported
dc.subject mitochondria eng
dc.subject OXPHOS system eng
dc.subject respiratory chain eng
dc.subject NADH dehydrogenase eng
dc.subject blue-native eng
dc.subject BN/SDS/SDS-PAGE eng
dc.subject Arabidopsis thaliana eng
dc.subject Mitochondrium ger
dc.subject Mitochondrion ger
dc.subject OXPHOS-System ger
dc.subject Oxidative Phosphorylierung ger
dc.subject Atmungskette ger
dc.subject NADH Dehydrogenase ger
dc.subject Arabidopsis thaliana ger
dc.subject Acker-Schmalwand ger
dc.subject Blue-Native ger
dc.subject.classification Mitochondrium ger
dc.subject.classification Oxidative Phosphorylierung ger
dc.subject.classification Atmungskette ger
dc.subject.classification NADH-Dehydrogenase ger
dc.subject.classification Ackerschmalwand ger
dc.subject.ddc 580 | Pflanzen (Botanik) ger
dc.title 3D Gel Map of Arabidopsis Complex I eng
dc.type Article
dc.type Text
dc.relation.issn 1664-462X
dc.bibliographicCitation.firstPage 153
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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