dc.identifier.uri |
http://dx.doi.org/10.15488/241 |
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dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/263 |
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dc.contributor.author |
Neuser, Frauke
|
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dc.contributor.author |
Zorn, Holger
|
|
dc.contributor.author |
Richter, Ulla
|
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dc.contributor.author |
Berger, Ralf Günter
|
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dc.date.accessioned |
2016-03-04T11:42:39Z |
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dc.date.available |
2016-03-04T11:42:39Z |
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dc.date.issued |
2000-04 |
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dc.identifier.citation |
Neuser, F.; Zorn, Holger; Richter, U.; Berger, R. G.: Purification, characterisation and cDNA sequencing of pyruvate decarboxylase from Zygosaccharomyces bisporus. In: Biological Chemistry 381 (2000), Nr. 4, S. 349-353. DOI: http://dx.doi.org/10.1515/BC.2000.046 |
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dc.description.abstract |
Cells of the wild-type yeast strain Zygosaccharomyces bisporus CBS 702 form alpha-hydroxy ketones from aromatic amino acid precursors during fermentation, Pyruvate decarboxylase (PDC, E.C. 4.1.1.1), the key enzyme of this biotransformation catalysing the nonoxidative decarboxylation of pyruvate and other 2-oxo-acids, was purified and characterised. The active enzyme is homotetrameric (alpha(4)) with a molecular mass of about 244 kDa, Activation of PDC by its substrate pyruvate results in a sigmoidal dependence of the reaction rate from substrate concentration (apparent K-m value 1.73 mM; Hill coefficient 2.10). A cDNA library was screened using a PCR-based procedure, and a 1856 bp cDNA of PDC was identified and sequenced. The cDNA encodes a polypeptide of 563 amino acid residues (monomeric unit), Sequence alignments demonstrate high homologies (> 80%) to PDC genes from Saccharomyces cerevisiae, Kluyveromyces lactis and Kluyveromyces marxianus. |
eng |
dc.description.sponsorship |
DFG |
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dc.language.iso |
eng |
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dc.publisher |
Berlin : Walter de Gruyter |
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dc.relation.ispartofseries |
Biological Chemistry 381 (2000), Nr. 4 |
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dc.rights |
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. |
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dc.subject |
brewers-yeast |
eng |
dc.subject |
pyrophosphate |
eng |
dc.subject |
binding |
eng |
dc.subject |
acyloin formation |
eng |
dc.subject |
enzyme purification |
eng |
dc.subject |
alpha-hydroxy ketones |
eng |
dc.subject |
non-conventional yeast |
eng |
dc.subject |
sequence alignment |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.subject.ddc |
580 | Pflanzen (Botanik)
|
ger |
dc.title |
Purification, characterisation and cDNA sequencing of pyruvate decarboxylase from Zygosaccharomyces bisporus |
eng |
dc.type |
Article |
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dc.type |
Text |
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dc.relation.issn |
1431-6730 |
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dc.relation.doi |
http://dx.doi.org/10.1515/BC.2000.046 |
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dc.bibliographicCitation.issue |
4 |
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dc.bibliographicCitation.volume |
381 |
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dc.bibliographicCitation.firstPage |
349 |
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dc.bibliographicCitation.lastPage |
353 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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