An extracellular carboxylesterase from the basidiomycete Pleurotus sapidus hydrolyses xanthophyll esters

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dc.identifier.uri http://dx.doi.org/10.15488/237
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/259
dc.contributor.author Zorn, Holger
dc.contributor.author Bouws, Henning
dc.contributor.author Takenberg, Meike
dc.contributor.author Nimtz, Manfred
dc.contributor.author Getzlaff, Rita
dc.contributor.author Breithaupt, Dietmar E.
dc.contributor.author Berger, Ralf Günter
dc.date.accessioned 2016-03-04T11:42:37Z
dc.date.available 2016-03-04T11:42:37Z
dc.date.issued 2005-05
dc.identifier.citation Zorn, Holger; Bouws, H.; Takenberg, M.; Nimtz, M.; Getzlaff, R.; Breithaupt, D. E.; Berger, R. G.: An extracellular carboxylesterase from the basidiomycete Pleurotus sapidus hydrolyses xanthophyll esters. In: Biological Chemistry 386 (2005), Nr. 5, S. 435-440. DOI: http://dx.doi.org/10.1515/BC.2005.052
dc.description.abstract An extracellular enzyme capable of efficient hydrolysis of xanthophyll esters was purified from culture supernatants of the basidiomycete Pleurotus sapidus. Under native conditions, the enzyme exhibited a molecular mass of 430 kDa, and SDS-PAGE data suggested a composition of eight identical subunits. Biochemical characterisation of the purified protein showed an isoelectric point of 4.5, and ideal hydrolysis conditions were observed at pH 5.8 and 40 degrees C. Partial amino acid sequences were derived from N-terminal Edman degradation and from mass spectrometric ab initio sequencing of internal peptides. An 1861-bp cDNA containing an open reading frame of 1641 bp was cloned from a cDNA library that showed ca. 40% homology to Candida rugosa lipases. The P sapidus carboxylesterase represents the first enzyme of the lipase/esterase family from a basidiomycetous fungus that has been characterised at the molecular level. eng
dc.language.iso eng
dc.publisher Berlin : Walter de Gruyter
dc.relation.ispartofseries Biological Chemistry 386 (2005), Nr. 5
dc.rights Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
dc.subject alpha/beta-hydrolase fold eng
dc.subject candida-rugosa lipases eng
dc.subject capsicum-annuum l. eng
dc.subject tagetes-erecta l. eng
dc.subject cholesterol esterase eng
dc.subject microbial lipases eng
dc.subject swiss-model eng
dc.subject proteins eng
dc.subject biotechnology eng
dc.subject carotenoids eng
dc.subject cDNA library eng
dc.subject fungi eng
dc.subject lipase eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 580 | Pflanzen (Botanik) ger
dc.title An extracellular carboxylesterase from the basidiomycete Pleurotus sapidus hydrolyses xanthophyll esters
dc.type article
dc.type Text
dc.relation.essn 1437-4315
dc.relation.issn 1431-6730
dc.relation.doi http://dx.doi.org/10.1515/BC.2005.052
dc.bibliographicCitation.issue 5
dc.bibliographicCitation.volume 386
dc.bibliographicCitation.firstPage 435
dc.bibliographicCitation.lastPage 440
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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