UV-resonance Raman spectroscopy of amino acids

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dc.identifier.uri http://dx.doi.org/10.15488/2534
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/2560
dc.contributor.author Höhl, Martin
dc.contributor.author Meinhardt-Wollweber, Merve
dc.contributor.author Schmitt, Heike
dc.contributor.author Lenarz, Thomas
dc.contributor.author Morgner, Uwe
dc.date.accessioned 2017-12-12T11:54:19Z
dc.date.available 2017-12-12T11:54:19Z
dc.date.issued 2016
dc.identifier.citation Höhl, M.; Meinhardt-Wollweber, M.; Schmitt, H.; Lenarz, T.; Morgner, U.: UV-resonance Raman spectroscopy of amino acids. In: Proceedings of SPIE 9704 (2016), 970404. DOI: https://doi.org/10.1117/12.2212781
dc.description.abstract Resonant enhancement of Raman signals is a useful method to increase sensitivity in samples with low concentration such as biological tissue. The investigation of resonance profiles shows the optimal excitation wavelength and yields valuable information about the molecules themselves. However careful characterization and calibration of all experimental parameters affecting quantum yield is required in order to achieve comparability of the single spectra recorded. We present an experimental technique for measuring the resonance profiles of different amino acids. The absorption lines of these molecules are located in the ultraviolet (UV) wavelength range. One limitation for broadband measurement of resonance profiles is the limited availability of Raman filters in certain regions of the UV for blocking the Rayleigh scattered light. Here, a wavelength range from 244.8 nm to 266.0 nm was chosen. The profiles reveal the optimal wavelength for recording the Raman spectra of amino acids in aqueous solutions in this range. This study provides the basis for measurements on more complex molecules such as proteins in the human perilymph. The composition of this liquid in the inner ear is essential for hearing and cannot be analyzed non-invasively so far. The long term aim is to implement this technique as a fiber based endoscope for non-invasive measurements during surgeries (e. g. cochlear implants) making it available as a diagnostic tool for physicians. This project is embedded in the interdisciplinary cluster of excellence "Hearing for all" (H4A). © 2016 SPIE. eng
dc.language.iso eng
dc.publisher Bellingham, WA : S P I E - International Society for Optical Engineering
dc.relation.ispartofseries Proceedings of SPIE 9704 (2016)
dc.rights Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
dc.subject amino acid eng
dc.subject perilymph eng
dc.subject Raman eng
dc.subject resonance eng
dc.subject uv eng
dc.subject.ddc 620 | Ingenieurwissenschaften und Maschinenbau ger
dc.title UV-resonance Raman spectroscopy of amino acids eng
dc.type article
dc.type conferenceObject
dc.type Text
dc.relation.issn 16057422
dc.relation.doi https://doi.org/10.1117/12.2212781
dc.bibliographicCitation.volume 9704
dc.bibliographicCitation.firstPage 970404
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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